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dc.contributor.authorPereira, Paulo S
dc.contributor.authorJones, Nic
dc.date.accessioned2009-11-10T09:55:49Z
dc.date.available2009-11-10T09:55:49Z
dc.date.issued2001-09
dc.identifier.citationThe RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating. 2001, 6 (9):789-802 Genes Cellsen
dc.identifier.issn1356-9597
dc.identifier.pmid11554925
dc.identifier.urihttp://hdl.handle.net/10541/85726
dc.description.abstractBACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshBase Sequence
dc.subject.meshBlotting, Northern
dc.subject.meshConjugation, Genetic
dc.subject.meshDNA Primers
dc.subject.meshFungal Proteins
dc.subject.meshGreen Fluorescent Proteins
dc.subject.meshHumans
dc.subject.meshLuminescent Proteins
dc.subject.meshMitogen-Activated Protein Kinase Kinases
dc.subject.meshMolecular Sequence Data
dc.subject.meshPeptides
dc.subject.meshPheromones
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRGS Proteins
dc.subject.meshRNA, Messenger
dc.subject.meshSchizosaccharomyces
dc.subject.meshSchizosaccharomyces pombe Proteins
dc.subject.meshSequence Homology, Amino Acid
dc.subject.meshSignal Transduction
dc.subject.meshTranscription Factors
dc.titleThe RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.en
dc.typeArticleen
dc.contributor.departmentImperial Cancer Research Fund, Gene Regulation Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.en
dc.identifier.journalGenes to Cellsen
html.description.abstractBACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.


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