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    The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.

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    Authors
    Pereira, Paulo S
    Jones, Nic
    Affiliation
    Imperial Cancer Research Fund, Gene Regulation Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
    Issue Date
    2001-09
    
    Metadata
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    Abstract
    BACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.
    Citation
    The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating. 2001, 6 (9):789-802 Genes Cells
    Journal
    Genes to Cells
    URI
    http://hdl.handle.net/10541/85726
    PubMed ID
    11554925
    Type
    Article
    Language
    en
    ISSN
    1356-9597
    Collections
    All Paterson Institute for Cancer Research

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