Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the Bracken carcinogen ptaquiloside.
Affiliation
Cancer Research Campaign Carcinogenesis Group, Paterson Institute for Cancer Research, Manchester M20 9BX, United Kingdom.Issue Date
2001-02-23
Metadata
Show full item recordAbstract
Following treatment with bracken fern (Pteridium aquilinum) extract and bracken spores a number of DNA adducts were detected by (32)P-postlabeling. Three of these adducts have been described previously (Povey et al., Br. J. Cancer (1996) 74, 1342-1348) and in this study, using a slightly different protocol, four new adducts, with higher chromatographic mobility, were detected at levels ranging from 50 to 230% of those previously described. When DNA was treated in vitro with activated ptaquiloside (APT) and analysed by butanol extraction or nuclease P1 treatment, only one adduct was detected by (32)P-postlabeling. This adduct was not present in the DNA from mice treated with bracken fern or spores, suggesting either that bracken contains genotoxins other than ptaquiloside or that the metabolism of ptaquiloside produces genotoxins not reflected by activated ptaquiloside. However, as the ATP-derived adduct has been detected previously in ileal DNA of bracken-fed calves, species-specific differences in the metabolism of bracken genotoxins may exist, thereby leading to differences in their biological outcomes.Citation
Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the Bracken carcinogen ptaquiloside. 2001, 281 (2):589-94 Biochem. Biophys. Res. Commun.Journal
Biochemical and Biophysical Research CommunicationsDOI
10.1006/bbrc.2001.4388PubMed ID
11181088Type
ArticleLanguage
enISSN
0006-291Xae974a485f413a2113503eed53cd6c53
10.1006/bbrc.2001.4388
Scopus Count
Collections
Related articles
- Assay of ptaquiloside, the carcinogenic principle of bracken, Pteridium aquilinum, by mutagenicity testing in Salmonella typhimurium.
- Authors: Matoba M, Saito E, Saito K, Koyama K, Natori S, Matsushima T, Takimoto M
- Issue date: 1987 Nov
- 32P-post-labelling analysis of DNA adducts formed in the upper gastrointestinal tissue of mice fed bracken extract or bracken spores.
- Authors: Povey AC, Potter D, O'Connor PJ
- Issue date: 1996 Nov
- Pteridium aquilinum and its ptaquiloside toxin induce DNA damage response in gastric epithelial cells, a link with gastric carcinogenesis.
- Authors: Gomes J, Magalhães A, Michel V, Amado IF, Aranha P, Ovesen RG, Hansen HC, Gärtner F, Reis CA, Touati E
- Issue date: 2012 Mar
- Ptaquiloside, the major carcinogen of bracken fern, in the pooled raw milk of healthy sheep and goats: an underestimated, global concern of food safety.
- Authors: Virgilio A, Sinisi A, Russo V, Gerardo S, Santoro A, Galeone A, Taglialatela-Scafati O, Roperto F
- Issue date: 2015 May 20
- Ptaquiloside in bracken spores from Britain.
- Authors: Rasmussen LH, Schmidt B, Sheffield E
- Issue date: 2013 Mar