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dc.contributor.authorDe Wynter, Erika A
dc.contributor.authorHeyworth, Clare M
dc.contributor.authorMukaida, Naofumi
dc.contributor.authorJaworska, Ewa
dc.contributor.authorWeffort-Santos, Almeriane
dc.contributor.authorMatushima, Kouji
dc.contributor.authorTesta, Nydia G
dc.date.accessioned2009-11-06T16:39:32Z
dc.date.available2009-11-06T16:39:32Z
dc.date.issued2001-09
dc.identifier.citationCCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors. 2001, 70 (3):455-60 J. Leukoc. Biol.en
dc.identifier.issn0741-5400
dc.identifier.pmid11527996
dc.identifier.urihttp://hdl.handle.net/10541/85589
dc.description.abstractSimple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.
dc.language.isoenen
dc.subjectFoetal Blooden
dc.subject.meshAntibodies
dc.subject.meshAntigens, CD34
dc.subject.meshBiological Markers
dc.subject.meshCell Culture Techniques
dc.subject.meshCell Differentiation
dc.subject.meshCell Lineage
dc.subject.meshCells, Cultured
dc.subject.meshChemokine CCL4
dc.subject.meshColony-Forming Units Assay
dc.subject.meshCulture Media, Serum-Free
dc.subject.meshErythroid Precursor Cells
dc.subject.meshFetal Blood
dc.subject.meshFlow Cytometry
dc.subject.meshGranulocytes
dc.subject.meshHumans
dc.subject.meshMacrophage Inflammatory Proteins
dc.subject.meshMacrophages
dc.subject.meshMyeloid Progenitor Cells
dc.subject.meshRNA, Messenger
dc.subject.meshReceptors, CCR1
dc.subject.meshReceptors, Chemokine
dc.titleCCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, United Kingdom. medeadw@leeds.ac.uken
dc.identifier.journalJournal of Leukocyte Biologyen
html.description.abstractSimple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.


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