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    CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors.

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    Authors
    De Wynter, Erika A
    Heyworth, Clare M
    Mukaida, Naofumi
    Jaworska, Ewa
    Weffort-Santos, Almeriane
    Matushima, Kouji
    Testa, Nydia G
    Affiliation
    Paterson Institute for Cancer Research, Manchester, United Kingdom. medeadw@leeds.ac.uk
    Issue Date
    2001-09
    
    Metadata
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    Abstract
    Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.
    Citation
    CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors. 2001, 70 (3):455-60 J. Leukoc. Biol.
    Journal
    Journal of Leukocyte Biology
    URI
    http://hdl.handle.net/10541/85589
    PubMed ID
    11527996
    Type
    Article
    Language
    en
    ISSN
    0741-5400
    Collections
    All Paterson Institute for Cancer Research

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