Show simple item record

dc.contributor.authorConnolly, Yvonne
dc.contributor.authorLittler, Edward
dc.contributor.authorSun, Ning
dc.contributor.authorChen, Xiaoyi
dc.contributor.authorHuang, Per-Chun
dc.contributor.authorStacey, Simon N
dc.contributor.authorArrand, John R
dc.date.accessioned2009-11-06T16:30:03Z
dc.date.available2009-11-06T16:30:03Z
dc.date.issued2001-03-01
dc.identifier.citationAntibodies to Epstein-Barr virus thymidine kinase: a characteristic marker for the serological detection of nasopharyngeal carcinoma. 2001, 91 (5):692-7 Int. J. Canceren
dc.identifier.issn0020-7136
dc.identifier.pmid11267982
dc.identifier.urihttp://hdl.handle.net/10541/85588
dc.description.abstractPatients suffering from nasopharyngeal carcinoma (NPC) generally exhibit elevated serum IgA antibody titres to Epstein-Barr virus (EBV) early antigen (EA) and virus capsid antigen (VCA). This property is frequently used as a diagnostic aid. Preliminary experiments suggested that an ELISA for IgA antibodies against the EBV-encoded thymidine kinase (TK) could form the basis of a more reliable diagnostic test. Here, we describe the construction of a recombinant baculovirus that expresses the EBV TK and present a full analysis of its use in serological surveys of NPC patients. Baculovirus-derived TK was used to develop a simple ELISA for serum IgA against this antigen. ELISA reactivity was strongly associated with NPC compared with an EBV-positive, normal control population. Comparison with the existing IgA-VCA and EA assays showed that the TK ELISA had higher sensitivity whilst the specificity was similar or higher. We conclude that the TK ELISA presents a strong predictor of NPC and, in its refined form, has improved pickup rates. In addition, results from patients with chronic nasopharyngitis (CNP) suggest that individuals with both symptoms of CNP and an elevated TK ELISA value may be at increased risk for the development of head-and-neck cancer.
dc.language.isoenen
dc.subjectNasopharyngeal Canceren
dc.subjectBiological Tumour Markersen
dc.subject.meshAnimals
dc.subject.meshAntibodies
dc.subject.meshBaculoviridae
dc.subject.meshCarcinoma
dc.subject.meshCell Line
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshHerpesvirus 4, Human
dc.subject.meshHumans
dc.subject.meshImmunoglobulin A
dc.subject.meshInsects
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshNasopharyngeal Neoplasms
dc.subject.meshNasopharyngitis
dc.subject.meshPilot Projects
dc.subject.meshRisk Factors
dc.subject.meshThymidine Kinase
dc.subject.meshTime Factors
dc.subject.meshTumor Markers, Biological
dc.titleAntibodies to Epstein-Barr virus thymidine kinase: a characteristic marker for the serological detection of nasopharyngeal carcinoma.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie CRC Research Centre, Manchester, United Kingdom.en
dc.identifier.journalInternational Journal of Cancer.en
html.description.abstractPatients suffering from nasopharyngeal carcinoma (NPC) generally exhibit elevated serum IgA antibody titres to Epstein-Barr virus (EBV) early antigen (EA) and virus capsid antigen (VCA). This property is frequently used as a diagnostic aid. Preliminary experiments suggested that an ELISA for IgA antibodies against the EBV-encoded thymidine kinase (TK) could form the basis of a more reliable diagnostic test. Here, we describe the construction of a recombinant baculovirus that expresses the EBV TK and present a full analysis of its use in serological surveys of NPC patients. Baculovirus-derived TK was used to develop a simple ELISA for serum IgA against this antigen. ELISA reactivity was strongly associated with NPC compared with an EBV-positive, normal control population. Comparison with the existing IgA-VCA and EA assays showed that the TK ELISA had higher sensitivity whilst the specificity was similar or higher. We conclude that the TK ELISA presents a strong predictor of NPC and, in its refined form, has improved pickup rates. In addition, results from patients with chronic nasopharyngitis (CNP) suggest that individuals with both symptoms of CNP and an elevated TK ELISA value may be at increased risk for the development of head-and-neck cancer.


This item appears in the following Collection(s)

Show simple item record