• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Relationships between clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine human cervix carcinoma cell lines.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Eastham, Angela M
    Atkinson, J
    West, Catharine M L
    Affiliation
    CRC Experimental Radiation Oncology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
    Issue Date
    2001-03
    
    Metadata
    Show full item record
    Abstract
    PURPOSE: To compare clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine cervix carcinoma cell lines. MATERIALS AND METHODS: Initial and residual (after 24h repair) radiation-induced DNA damage was evaluated using pulsed field gel electrophoresis. Chromosome damage was measured by micronucleus (MN) induction in cytochalasin-B-induced binucleate cells. RESULTS: Significant differences between the cell lines were obtained in the induced levels of initial damage, residual damage and MN. Values for SF2 for the nine cell lines ranged from 0.36 to 0.92. No correlation was found between clonogenic measurements of radiosensitivity and initial DNA damage dose response slopes. However, borderline significant correlations were seen between clonogenic radiosensitivity data and the levels of residual DNA damage. There was no correlation between clonogenic radiosensitivity and the levels of radiation-induced MN. Cell lines with high levels of initial damage had high yields of MN induced by radiation and the correlation seen was significant. CONCLUSIONS: The poor correlation between the different endpoints precludes their use in a clinical setting on primary tumour samples in vitro. It may be that tumour cell lines in vitro are a poor model for tumours in vivo. Studies aimed at assessing assays for measuring tumour radiosensitivity therefore should employ clinical samples. In vitro cell line work should concentrate on unravelling the complex mechanisms involved in determining a radiosensitive or radioresistant phenotype.
    Citation
    Relationships between clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine human cervix carcinoma cell lines. 2001, 77 (3):295-302 Int. J. Radiat. Biol.
    Journal
    International Journal of Radiation Biology
    URI
    http://hdl.handle.net/10541/85579
    DOI
    10.1080/09553000010017108
    PubMed ID
    11258843
    Type
    Article
    Language
    en
    ISSN
    0955-3002
    ae974a485f413a2113503eed53cd6c53
    10.1080/09553000010017108
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • A correlation between residual DNA double-strand breaks and clonogenic measurements of radiosensitivity in fibroblasts from preradiotherapy cervix cancer patients.
    • Authors: Kiltie AE, Orton CJ, Ryan AJ, Roberts SA, Marples B, Davidson SE, Hunter RD, Margison GP, West CM, Hendry JH
    • Issue date: 1997 Dec 1
    • Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters.
    • Authors: Eastham AM, Marples B, Kiltie AE, Orton CJ, West CM
    • Issue date: 1999 Mar
    • The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines.
    • Authors: Marples B, Longhurst D, Eastham AM, West CM
    • Issue date: 1998 Apr
    • Potential application of the ATP cell viability assay in the measurement of intrinsic radiosensitivity in cervical cancer.
    • Authors: Tam KF, Ng TY, Liu SS, Tsang PC, Kwong PW, Ngan HY
    • Issue date: 2005 Mar
    • Intrinsic Radiosensitivity and Cellular Characterization of 27 Canine Cancer Cell Lines.
    • Authors: Maeda J, Froning CE, Brents CA, Rose BJ, Thamm DH, Kato TA
    • Issue date: 2016
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.