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dc.contributor.authorVivès, Romain R
dc.contributor.authorGoodger, Sarah J
dc.contributor.authorPye, David A
dc.date.accessioned2009-11-06T14:47:31Z
dc.date.available2009-11-06T14:47:31Z
dc.date.issued2001-02-15
dc.identifier.citationCombined strong anion-exchange HPLC and PAGE approach for the purification of heparan sulphate oligosaccharides. 2001, 354 (Pt 1):141-7 Biochem. J.en
dc.identifier.issn0264-6021
dc.identifier.pmid11171089
dc.identifier.urihttp://hdl.handle.net/10541/85563
dc.description.abstractHeparan sulphates are highly sulphated linear polysaccharides involved in many cellular functions. Their biological properties stem from their ability to interact with a wide range of proteins. An increasing number of studies, using heparan sulphate-derived oligosaccharides, suggest that specific structural features within the polysaccharide are responsible for ligand recognition and regulation. In the present study, we show that strong anion-exchange HPLC alone, a commonly used technique for purification of heparan sulphate-derived oligosaccharides, may not permit the isolation of highly pure heparan sulphate oligosaccharide species. This was determined by PAGE analysis of hexa-, octa- and decasaccharide samples deemed to be pure by strong anion-exchange HPLC. In addition, subtle differences in the positioning of sulphate groups within heparan sulphate hexasaccharides were impossible to detect by strong anion-exchange HPLC. PAGE analysis on the other hand afforded excellent resolution of these structural isomers. The precise positioning of specific sulphate groups has been implicated in determining the specificity of heparan sulphate interactions and biological activities; hence, the purification of oligosaccharide species that differ in this way becomes an important issue. In this study, we have used strong anion-exchange HPLC and PAGE techniques to allow production of the homogeneous heparan sulphate oligosaccharide species that will be required for the detailed study of structure/activity relationships.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshChromatography, Ion Exchange
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshHeparitin Sulfate
dc.subject.meshSwine
dc.titleCombined strong anion-exchange HPLC and PAGE approach for the purification of heparan sulphate oligosaccharides.en
dc.typeArticleen
dc.contributor.departmentCRC Drug Development Group, and University of Manchester Department of Medical Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester M20 4BX, UK.en
dc.identifier.journalThe Biochemical Journalen
html.description.abstractHeparan sulphates are highly sulphated linear polysaccharides involved in many cellular functions. Their biological properties stem from their ability to interact with a wide range of proteins. An increasing number of studies, using heparan sulphate-derived oligosaccharides, suggest that specific structural features within the polysaccharide are responsible for ligand recognition and regulation. In the present study, we show that strong anion-exchange HPLC alone, a commonly used technique for purification of heparan sulphate-derived oligosaccharides, may not permit the isolation of highly pure heparan sulphate oligosaccharide species. This was determined by PAGE analysis of hexa-, octa- and decasaccharide samples deemed to be pure by strong anion-exchange HPLC. In addition, subtle differences in the positioning of sulphate groups within heparan sulphate hexasaccharides were impossible to detect by strong anion-exchange HPLC. PAGE analysis on the other hand afforded excellent resolution of these structural isomers. The precise positioning of specific sulphate groups has been implicated in determining the specificity of heparan sulphate interactions and biological activities; hence, the purification of oligosaccharide species that differ in this way becomes an important issue. In this study, we have used strong anion-exchange HPLC and PAGE techniques to allow production of the homogeneous heparan sulphate oligosaccharide species that will be required for the detailed study of structure/activity relationships.


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