Role of heparan sulfate-2-O-sulfotransferase in the mouse.
dc.contributor.author | Merry, Catherine L R | |
dc.contributor.author | Wilson, Valerie A | |
dc.date.accessioned | 2009-11-06T09:15:26Z | |
dc.date.available | 2009-11-06T09:15:26Z | |
dc.date.issued | 2002-12-19 | |
dc.identifier.citation | Role of heparan sulfate-2-O-sulfotransferase in the mouse. 2002, 1573 (3):319-27 Biochim. Biophys. Acta | en |
dc.identifier.issn | 0006-3002 | |
dc.identifier.pmid | 12417414 | |
dc.identifier.uri | http://hdl.handle.net/10541/85497 | |
dc.description.abstract | Heparan sulfate (HS) is a long unbranched polysaccharide found covalently attached to various proteins at the cell surface and in the extracellular matrix. It plays a central role in embryonic development and cellular function by modulating the activities of an extensive range of growth factors and morphogens. HS 2-O-sulfotransferase (Hs2st) occupies a critical position in the succession of enzymes responsible for the biosynthesis of HS, catalysing the transfer of sulfate to the C2-position of selected hexuronic acid residues within the nascent HS chain. Previous studies have concluded that 2-O-sulfation of HS is essential for it to cooperate in many growth factor/receptor interactions. Surprisingly therefore, embryos lacking functional Hs2st survive until birth, but die perinatally, suffering complete failure to form kidneys. However, this rather late lethality belies a more intricate involvement of 2-O-sulfated HS during development. The purpose of this review is to summarise the requirements for 2-O-sulfated HS during mouse development, at the morphological and molecular level. The implications that altered HS structure may have on growth factor/receptor signalling in vivo will be discussed. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Growth Substances | |
dc.subject.mesh | Heparitin Sulfate | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Phenotype | |
dc.subject.mesh | Receptors, Growth Factor | |
dc.subject.mesh | Signal Transduction | |
dc.subject.mesh | Sulfotransferases | |
dc.title | Role of heparan sulfate-2-O-sulfotransferase in the mouse. | en |
dc.type | Article | en |
dc.contributor.department | Cancer Research Campaign, Department of Medical Oncology, Christie Hospital NHS Trust, Manchester, United Kingdom. | en |
dc.identifier.journal | Biochimica et Biophysica Acta | en |
html.description.abstract | Heparan sulfate (HS) is a long unbranched polysaccharide found covalently attached to various proteins at the cell surface and in the extracellular matrix. It plays a central role in embryonic development and cellular function by modulating the activities of an extensive range of growth factors and morphogens. HS 2-O-sulfotransferase (Hs2st) occupies a critical position in the succession of enzymes responsible for the biosynthesis of HS, catalysing the transfer of sulfate to the C2-position of selected hexuronic acid residues within the nascent HS chain. Previous studies have concluded that 2-O-sulfation of HS is essential for it to cooperate in many growth factor/receptor interactions. Surprisingly therefore, embryos lacking functional Hs2st survive until birth, but die perinatally, suffering complete failure to form kidneys. However, this rather late lethality belies a more intricate involvement of 2-O-sulfated HS during development. The purpose of this review is to summarise the requirements for 2-O-sulfated HS during mouse development, at the morphological and molecular level. The implications that altered HS structure may have on growth factor/receptor signalling in vivo will be discussed. |