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dc.contributor.authorElkord, Eyad
dc.date.accessioned2009-11-03T12:44:59Z
dc.date.available2009-11-03T12:44:59Z
dc.date.issued2009-08-15
dc.identifier.citationFrequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. 2009, 347 (1-2):87-90 J. Immunol. Methodsen
dc.identifier.issn1872-7905
dc.identifier.pmid19538964
dc.identifier.doi10.1016/j.jim.2009.06.001
dc.identifier.urihttp://hdl.handle.net/10541/85219
dc.description.abstractCryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.
dc.language.isoenen
dc.subject.meshCD4 Lymphocyte Count
dc.subject.meshCell Separation
dc.subject.meshCryopreservation
dc.subject.meshFlow Cytometry
dc.subject.meshForkhead Transcription Factors
dc.subject.meshHumans
dc.subject.meshInterleukin-2 Receptor alpha Subunit
dc.subject.meshReference Values
dc.subject.meshT-Lymphocyte Subsets
dc.subject.meshT-Lymphocytes, Regulatory
dc.titleFrequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation.en
dc.typeArticleen
dc.contributor.departmentClinical Immunotherapy Laboratory, Department of Medical Oncology, University of Manchester, Wilmslow Road, Manchester M204BX, UK. eelkord@picr.man.ac.uken
dc.identifier.journalJournal of Immunological Methodsen
html.description.abstractCryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.


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