Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation.
Authors
Elkord, EyadAffiliation
Clinical Immunotherapy Laboratory, Department of Medical Oncology, University of Manchester, Wilmslow Road, Manchester M204BX, UK. eelkord@picr.man.ac.ukIssue Date
2009-08-15
Metadata
Show full item recordAbstract
Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.Citation
Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. 2009, 347 (1-2):87-90 J. Immunol. MethodsJournal
Journal of Immunological MethodsDOI
10.1016/j.jim.2009.06.001PubMed ID
19538964Type
ArticleLanguage
enISSN
1872-7905ae974a485f413a2113503eed53cd6c53
10.1016/j.jim.2009.06.001
Scopus Count
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