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dc.contributor.authorPotten, Christopher S
dc.contributor.authorBooth, Dawn
dc.contributor.authorCragg, N J
dc.contributor.authorO'Shea, Julie A
dc.contributor.authorTudor, Gregory L
dc.contributor.authorBooth, Catherine
dc.date.accessioned2009-10-19T15:34:03Z
dc.date.available2009-10-19T15:34:03Z
dc.date.issued2002-08
dc.identifier.citationCell kinetic studies in murine ventral tongue epithelium: cell cycle progression studies using double labelling techniques. 2002, 35 Suppl 1:16-21 Cell Prolif.en
dc.identifier.issn0960-7722
dc.identifier.pmid12139704
dc.identifier.doi10.1046/j.1365-2184.35.s1.2.x
dc.identifier.urihttp://hdl.handle.net/10541/84407
dc.description.abstractThe dorsal and ventral epithelia on the murine tongue exhibit very pronounced circadian rhythms in terms of the cell cycle. These rhythms are such that three injections of tritiated thymidine 3 h apart spanning the circadian peak in S phase cells labelled between 40 and 50% of the basal cells. Injection of bromodeoxyuridine generally gave slightly lower labelling indices. Approximately the same proportion (54% of the basal cells) could be accumulated in metaphase over a 24-h period using vincristine as a stathmokinetic agent. The experiments reported here using mouse ventral tongue epithelium use double-labelling approaches to address the question: what proportion of the approximately 50% of the basal cells that are proliferating have a 24-h cell cycle and can therefore be labelled by a similar labelling protocol the following day? The results suggest a heterogeneity amongst the proliferating basal cells, similar to the heterogeneity proposed for the dorsal tongue epithelium. Although not all the basal component has been accounted for, the data presented here suggest that about 20% of the basal cells may have a cell cycle time of 24 h, about 30% appear to have a longer cell cycle time (48 or 72 h), while about 20% of the basal cells appear to be postmitotic maturing G1 cells, awaiting the appropriate signals for migration into the suprabasal layer.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntimetabolites
dc.subject.meshAntineoplastic Agents, Phytogenic
dc.subject.meshBromodeoxyuridine
dc.subject.meshCell Division
dc.subject.meshEpithelial Cells
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMitosis
dc.subject.meshThymidine
dc.subject.meshTongue
dc.subject.meshTritium
dc.subject.meshVincristine
dc.titleCell kinetic studies in murine ventral tongue epithelium: cell cycle progression studies using double labelling techniques.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.en
dc.identifier.journalCell Proliferationen
html.description.abstractThe dorsal and ventral epithelia on the murine tongue exhibit very pronounced circadian rhythms in terms of the cell cycle. These rhythms are such that three injections of tritiated thymidine 3 h apart spanning the circadian peak in S phase cells labelled between 40 and 50% of the basal cells. Injection of bromodeoxyuridine generally gave slightly lower labelling indices. Approximately the same proportion (54% of the basal cells) could be accumulated in metaphase over a 24-h period using vincristine as a stathmokinetic agent. The experiments reported here using mouse ventral tongue epithelium use double-labelling approaches to address the question: what proportion of the approximately 50% of the basal cells that are proliferating have a 24-h cell cycle and can therefore be labelled by a similar labelling protocol the following day? The results suggest a heterogeneity amongst the proliferating basal cells, similar to the heterogeneity proposed for the dorsal tongue epithelium. Although not all the basal component has been accounted for, the data presented here suggest that about 20% of the basal cells may have a cell cycle time of 24 h, about 30% appear to have a longer cell cycle time (48 or 72 h), while about 20% of the basal cells appear to be postmitotic maturing G1 cells, awaiting the appropriate signals for migration into the suprabasal layer.


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