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dc.contributor.authorPotten, Christopher S
dc.contributor.authorBooth, Dawn
dc.contributor.authorCragg, N J
dc.contributor.authorO'Shea, Julie A
dc.contributor.authorTudor, Gregory L
dc.contributor.authorBooth, Catherine
dc.date.accessioned2009-10-16T14:54:06Z
dc.date.available2009-10-16T14:54:06Z
dc.date.issued2002-08
dc.identifier.citationCell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor. 2002, 35 Suppl 1:22-31 Cell Prolif.en
dc.identifier.issn0960-7722
dc.identifier.pmid12139705
dc.identifier.doi10.1046/j.1365-2184.35.s1.3.x
dc.identifier.urihttp://hdl.handle.net/10541/84350
dc.description.abstractKeratinocyte growth factor (KGF) stimulates proliferation and differentiation in various epithelial systems. Three daily subcutaneous injections of 125 microg of this protein into mice induce dramatic changes in the histology and histometric measurements of the ventral tongue epithelium. The thickness of the epithelium is increased two-fold and the number of cells beneath a 1-mm length of the surface is increased 1.6-fold. KGF also induces a four-fold increase in the number of S phase cells labelled with tritiated thymidine in the basal layer on the third day after KGF administration. The increase in thickness and cellularity persist for at least 4 days after the end of the KGF injections. However, there is a dramatic fall in the number of S phase cells detected by 3HTdR pulse labelling 2 days after the end of the KGF treatment. There are indications that by 7 days after the 3-day regimen of KGF treatment, both thickness and cellularity have fallen back to near control levels. Continued exposure to KGF over a period of 7 days does not result in any further increases in thickness, cellularity or proliferation. In fact, the proliferation decreases on the fifth, sixth and seventh days of KGF injection to control values on day 7. These changes in the epithelium following KGF treatment suggest that the thicker and more cellular epithelium may be more able to cope with an exposure to a cytotoxic agent and hence be protected in comparison with normal or vehicle-treated epithelium.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Division
dc.subject.meshEpithelial Cells
dc.subject.meshFibroblast Growth Factor 7
dc.subject.meshFibroblast Growth Factors
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshThymidine
dc.subject.meshTongue
dc.subject.meshTritium
dc.titleCell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.en
dc.identifier.journalCell Proliferationen
html.description.abstractKeratinocyte growth factor (KGF) stimulates proliferation and differentiation in various epithelial systems. Three daily subcutaneous injections of 125 microg of this protein into mice induce dramatic changes in the histology and histometric measurements of the ventral tongue epithelium. The thickness of the epithelium is increased two-fold and the number of cells beneath a 1-mm length of the surface is increased 1.6-fold. KGF also induces a four-fold increase in the number of S phase cells labelled with tritiated thymidine in the basal layer on the third day after KGF administration. The increase in thickness and cellularity persist for at least 4 days after the end of the KGF injections. However, there is a dramatic fall in the number of S phase cells detected by 3HTdR pulse labelling 2 days after the end of the KGF treatment. There are indications that by 7 days after the 3-day regimen of KGF treatment, both thickness and cellularity have fallen back to near control levels. Continued exposure to KGF over a period of 7 days does not result in any further increases in thickness, cellularity or proliferation. In fact, the proliferation decreases on the fifth, sixth and seventh days of KGF injection to control values on day 7. These changes in the epithelium following KGF treatment suggest that the thicker and more cellular epithelium may be more able to cope with an exposure to a cytotoxic agent and hence be protected in comparison with normal or vehicle-treated epithelium.


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