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    Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.

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    Authors
    Potten, Christopher S
    Booth, Dawn
    Cragg, N J
    O'Shea, Julie A
    Tudor, Gregory L
    Booth, Catherine
    Affiliation
    Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.
    Issue Date
    2002-08
    
    Metadata
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    Abstract
    Keratinocyte growth factor (KGF) stimulates proliferation and differentiation in various epithelial systems. Three daily subcutaneous injections of 125 microg of this protein into mice induce dramatic changes in the histology and histometric measurements of the ventral tongue epithelium. The thickness of the epithelium is increased two-fold and the number of cells beneath a 1-mm length of the surface is increased 1.6-fold. KGF also induces a four-fold increase in the number of S phase cells labelled with tritiated thymidine in the basal layer on the third day after KGF administration. The increase in thickness and cellularity persist for at least 4 days after the end of the KGF injections. However, there is a dramatic fall in the number of S phase cells detected by 3HTdR pulse labelling 2 days after the end of the KGF treatment. There are indications that by 7 days after the 3-day regimen of KGF treatment, both thickness and cellularity have fallen back to near control levels. Continued exposure to KGF over a period of 7 days does not result in any further increases in thickness, cellularity or proliferation. In fact, the proliferation decreases on the fifth, sixth and seventh days of KGF injection to control values on day 7. These changes in the epithelium following KGF treatment suggest that the thicker and more cellular epithelium may be more able to cope with an exposure to a cytotoxic agent and hence be protected in comparison with normal or vehicle-treated epithelium.
    Citation
    Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor. 2002, 35 Suppl 1:22-31 Cell Prolif.
    Journal
    Cell Proliferation
    URI
    http://hdl.handle.net/10541/84350
    DOI
    10.1046/j.1365-2184.35.s1.3.x
    PubMed ID
    12139705
    Type
    Article
    Language
    en
    ISSN
    0960-7722
    ae974a485f413a2113503eed53cd6c53
    10.1046/j.1365-2184.35.s1.3.x
    Scopus Count
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    All Paterson Institute for Cancer Research

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