Cell kinetic studies in the murine ventral tongue epithelium: mucositis induced by radiation and its protection by pretreatment with keratinocyte growth factor (KGF).
Authors
Potten, Christopher SBooth, Dawn
Cragg, N J
Tudor, Gregory L
O'Shea, Julie A
Booth, Catherine
Meineke, F A
Barthel, D
Loeffler, M
Affiliation
Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.Issue Date
2002-08
Metadata
Show full item recordAbstract
Radiation kills or reduces reproductive capacity of proliferating cells, including stem cells. In the oral mucosae this can result in a decline in the number of cells in the tissue which, if severe enough, will result in the formation of an ulcer when the cellularity essentially reaches zero. We have used histometric measurements of cellularity following exposure to radiation in mouse ventral tongue epithelium as a model for oral mucositis (ulcer development). Here we provide further measurements of cellularity changes in the basal layer and in the epithelium as a whole at various times following 15, 20 or 25 Gy doses. The protective effects of prior treatment with keratinocyte growth factor (KGF) are also investigated. 20 Gy of 300 kV X-rays has become our standard reference dose and the changes in cellularity seen following this dose are highly reproducible, with minimum values being observed 6 days following irradiation. A higher dose results in a greater reduction of cellularity, although the minimum value also occurs at 6 days. A lower dose (15 Gy) results in a much shallower curve, with a minimum value being observed about 1 day earlier. These changes in cellularity can be related to the less sensitive index of mucositis, namely epithelial thickness. There is also a sharp peak in proliferation about 1 day after the minimum in cellularity, i.e. on day 7. The peak following a lower dose of radiation occurs a little earlier and, following the higher dose, the peak tends to be broader. Previous work and data presented in the preceding paper in this series has shown that KGF, given over a period of 3 days, results in a dramatic increase in epithelial thickness in oral mucosa, including the ventral tongue. As a result of the increased cellularity induced by KGF given before radiation, a delay in the fall in cellularity results, which is the consequence of the increased number of cells in the epithelium at the beginning of the study.Citation
Cell kinetic studies in the murine ventral tongue epithelium: mucositis induced by radiation and its protection by pretreatment with keratinocyte growth factor (KGF). 2002, 35 Suppl 1:32-47 Cell Prolif.Journal
Cell ProliferationDOI
10.1046/j.1365-2184.35.s1.4.xPubMed ID
12139706Type
ArticleLanguage
enISSN
0960-7722ae974a485f413a2113503eed53cd6c53
10.1046/j.1365-2184.35.s1.4.x
Scopus Count
Collections
Related articles
- Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.
- Authors: Potten CS, Booth D, Cragg NJ, O'Shea JA, Tudor GL, Booth C
- Issue date: 2002 Aug
- The effect of keratinocyte growth factor on healing of manifest radiation ulcers in mouse tongue epithelium.
- Authors: Dörr W, Spekl K, Farrell CL
- Issue date: 2002 Aug
- The effects of keratinocyte growth factor in preclinical models of mucositis.
- Authors: Farrell CL, Rex KL, Chen JN, Bready JV, DiPalma CR, Kaufman SA, Rattan A, Scully S, Lacey DL
- Issue date: 2002 Aug
- A quantitative histometric murine in vivo model of radiation-induced oral mucositis.
- Authors: Wardley AM, Booth D, Roberts SA, Scarffe JH, Potten CS
- Issue date: 1998 Jul
- Modification of oral mucositis by keratinocyte growth factor: single radiation exposure.
- Authors: Dörr W, Noack R, Spekl K, Farrell CL
- Issue date: 2001 Mar