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    Cell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination.

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    Authors
    Potten, Christopher S
    Booth, Dawn
    Cragg, N J
    Tudor, Gregory L
    O'Shea, Julie A
    Appleton, D R
    Barthel, D
    Gerike, T G
    Meineke, F A
    Loeffler, M
    Booth, Catherine
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    Affiliation
    Paterson Institute for cancer Research, Christie Hospital NHS Trust, wilmslow Road, Manchester M20 4BX, UK.
    Issue Date
    2002-08
    
    Metadata
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    Abstract
    The oral mucosa is a rapidly replacing body tissue that has received relatively little attention in terms of defining its cell kinetics and cellular organization. The tissue is sensitive to the effects of cytotoxic agents, the consequence of which can be stem cell death with the subsequent development of ulcers and the symptoms of oral mucositis. There is considerable interest in designing strategies to protect oral stem cells and, hence, reduce the mucositis side-effects in cancer therapy patients. Here we present details of a new histometric approach designed to investigate the changing patterns in cellularity in the ventral tongue mucosa. This initial paper in a series of four papers presents observations on the changing patterns in the labelling index following tritiated thymidine administration, which suggest a delayed uptake of tritiated thymidine from a long-term intracellular thymidine pool, a phenomenon that will complicate cell kinetic interpretations in a variety of experimental situations. We also provide data on the changing pattern of mitotic activity through a 24-h period (circadian rhythms). Using vincristine-induced stathmokinesis, the data indicate that 54% of the basal cells divide each day and that there is a high degree of synchrony in mitotic activity with a mitotic peak occurring around 13.00 h. The mitotic circadian peak occurs 9-12 h after the circadian peak in DNA synthesis. The data presented here and in the subsequent papers could be interpreted to indicate that basal cells of BDF1 mice have an average turnover time of about 26-44 h with some cells cycling once a day and others with a 2- or 3-day cell cycle time.
    Citation
    Cell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination. 2002, 35 Suppl 1:1-15 Cell Prolif.
    Journal
    Cell Proliferation
    URI
    http://hdl.handle.net/10541/84323
    DOI
    10.1046/j.1365-2184.35.s1.1.x
    PubMed ID
    12139703
    Type
    Article
    Language
    en
    ISSN
    0960-7722
    ae974a485f413a2113503eed53cd6c53
    10.1046/j.1365-2184.35.s1.1.x
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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