Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.
Findlay, Victoria J
Millar, Jonathan B A
Morgan, Brian A
Toone, W Mark
AffiliationCancer Research UK Cell Regulation Group, Paterson Institute for Cancer Research. Christie Hospital, Withington, Manchester M20 4BX, UK
MetadataShow full item record
AbstractThe signaling pathways that sense adverse stimuli and communicate with the nucleus to initiate appropriate changes in gene expression are central to the cellular stress response. Herein, we have characterized the role of the Sty1 (Spc1) stress-activated mitogen-activated protein kinase pathway, and the Pap1 and Atf1 transcription factors, in regulating the response to H(2)O(2) in the fission yeast Schizosaccharomyces pombe. We find that H(2)O(2) activates the Sty1 pathway in a dose-dependent manner via at least two sensing mechanisms. At relatively low levels of H(2)O(2), a two component-signaling pathway, which feeds into either of the two stress-activated mitogen-activated protein kinase kinase kinases Wak1 or Win1, regulates Sty1 phosphorylation. In contrast, at high levels of H(2)O(2), Sty1 activation is controlled predominantly by a two-component independent mechanism and requires the function of both Wak1 and Win1. Individual transcription factors were also found to function within a limited range of H(2)O(2) concentrations. Pap1 activates target genes primarily in response to low levels of H(2)O(2), whereas Atf1 primarily controls the transcriptional response to high concentrations of H(2)O(2). Our results demonstrate that S. pombe uses a combination of stress-responsive regulatory proteins to gauge and effect the appropriate transcriptional response to increasing concentrations of H(2)O(2).
CitationDistinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe. 2002, 13 (3):805-16 Mol. Biol. Cell
JournalMolecular Biology of the Cell
- Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation.
- Authors: Salat-Canela C, Paulo E, Sánchez-Mir L, Carmona M, Ayté J, Oliva B, Hidalgo E
- Issue date: 2017 Aug 18
- Activation of the redox sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration.
- Authors: Vivancos AP, Castillo EA, Jones N, Ayté J, Hidalgo E
- Issue date: 2004 Jun
- Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.
- Authors: Toone WM, Kuge S, Samuels M, Morgan BA, Toda T, Jones N
- Issue date: 1998 May 15
- Regulation of Schizosaccharomyces pombe Atf1 protein levels by Sty1-mediated phosphorylation and heterodimerization with Pcr1.
- Authors: Lawrence CL, Maekawa H, Worthington JL, Reiter W, Wilkinson CR, Jones N
- Issue date: 2007 Feb 23
- Oxidation of a eukaryotic 2-Cys peroxiredoxin is a molecular switch controlling the transcriptional response to increasing levels of hydrogen peroxide.
- Authors: Bozonet SM, Findlay VJ, Day AM, Cameron J, Veal EA, Morgan BA
- Issue date: 2005 Jun 17