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dc.contributor.authorSummers, Yvonne J
dc.contributor.authorHeyworth, Clare M
dc.contributor.authorDe Wynter, Erika A
dc.contributor.authorChang, James
dc.contributor.authorTesta, Nydia G
dc.date.accessioned2009-10-13T10:38:44Z
dc.date.available2009-10-13T10:38:44Z
dc.date.issued2001
dc.identifier.citationCord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells. 2001, 19 (6):505-13 Stem Cellsen
dc.identifier.issn1066-5099
dc.identifier.pmid11713342
dc.identifier.doi10.1634/stemcells.19-6-505
dc.identifier.urihttp://hdl.handle.net/10541/84158
dc.description.abstractWe examined the functional differences between G(0) and G(1) cord blood CD34+ cells for up to 24 weeks in serum-free suspension culture, containing Flt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x 10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34+, resulting in a 600-fold expansion of CD34+ cells at week 8. As in other in vitro systems, total cell expansion is less discriminatory of stem cell behavior than progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34
dc.subject.meshCell Count
dc.subject.meshCell Cycle
dc.subject.meshCell Division
dc.subject.meshFetal Blood
dc.subject.meshFlow Cytometry
dc.subject.meshG0 Phase
dc.subject.meshG1 Phase
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshTime Factors
dc.titleCord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells.en
dc.typeArticleen
dc.contributor.departmentCRC Experimental Haematology Group, Paterson Institute for Cancer Research, Manchester MZ0 4BX, United Kingdom. ysummers@picr.man.ac.uken
dc.identifier.journalStem Cellsen
html.description.abstractWe examined the functional differences between G(0) and G(1) cord blood CD34+ cells for up to 24 weeks in serum-free suspension culture, containing Flt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x 10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34+, resulting in a 600-fold expansion of CD34+ cells at week 8. As in other in vitro systems, total cell expansion is less discriminatory of stem cell behavior than progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks.


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