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dc.contributor.authorDehal, P K
dc.contributor.authorEmbleton, Jim
dc.contributor.authorKemshead, John T
dc.contributor.authorHawkins, Robert E
dc.date.accessioned2009-10-05T15:16:38Z
dc.date.available2009-10-05T15:16:38Z
dc.date.issued2002-08
dc.identifier.citationTargeted cytokine delivery to neuroblastoma. 2002, 30 (4):518-20 Biochem. Soc. Trans.en
dc.identifier.issn0300-5127
dc.identifier.pmid12196127
dc.identifier.doi10.1042/
dc.identifier.urihttp://hdl.handle.net/10541/83572
dc.description.abstractThe aim of this study was to construct a fusion protein from the cytokine granulocyte/macrophage colony-stimulating factor (GM-CSF) and a single-chain Fv fragment (scFv D29) and to investigate its potential to activate cells of the immune system against neuroblastoma cells expressing neural cell adhesion molecule (NCAM). Mammalian cell expression of the scFv D29-GM-CSF fusion protein was compared using a number of vectors, including retroviral and adenoviral vectors. The resultant fusion protein, expressed by HeLa cells, was found by ELISA to bind immobilized recombinant NCAM. Moreover, FACS analysis confirmed binding to the human neuroblastoma cell line SKNBE and a murine neuroblastoma cell line engineered to express the glycosylphosphatidylinositol form of human NCAM (N2A-rKNIE). The fusion protein was also found to stimulate the proliferation of the FDC-P1 haemopoietic cell line, which is dependent on GM-CSF (or interleukin 3) for continued growth. In vitro clonogenic assays indicated that scFv-GM-CSF could selectively induce growth inhibition of SKNBE cells by murine lymphoid cells.
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals
dc.subject.meshCytokines
dc.subject.meshHumans
dc.subject.meshImmunotherapy
dc.subject.meshInterleukin-2
dc.subject.meshMammals
dc.subject.meshNeuroblastoma
dc.subject.meshRecombinant Proteins
dc.subject.meshTumor Cells, Cultured
dc.titleTargeted cytokine delivery to neuroblastoma.en
dc.typeArticleen
dc.contributor.departmentMedical Oncology, Paterson Institute for Cancer Research, Wilmslow Road, Withington, Manchester M20 4BX, UK.en
dc.identifier.journalBiochemical Society Transactionsen
html.description.abstractThe aim of this study was to construct a fusion protein from the cytokine granulocyte/macrophage colony-stimulating factor (GM-CSF) and a single-chain Fv fragment (scFv D29) and to investigate its potential to activate cells of the immune system against neuroblastoma cells expressing neural cell adhesion molecule (NCAM). Mammalian cell expression of the scFv D29-GM-CSF fusion protein was compared using a number of vectors, including retroviral and adenoviral vectors. The resultant fusion protein, expressed by HeLa cells, was found by ELISA to bind immobilized recombinant NCAM. Moreover, FACS analysis confirmed binding to the human neuroblastoma cell line SKNBE and a murine neuroblastoma cell line engineered to express the glycosylphosphatidylinositol form of human NCAM (N2A-rKNIE). The fusion protein was also found to stimulate the proliferation of the FDC-P1 haemopoietic cell line, which is dependent on GM-CSF (or interleukin 3) for continued growth. In vitro clonogenic assays indicated that scFv-GM-CSF could selectively induce growth inhibition of SKNBE cells by murine lymphoid cells.


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