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    Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues.

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    Authors
    Castillo, Esther A
    Ayte, Jose
    Chiva, Cristina
    Moldón, Alberto
    Carrascal, Montse
    Abián, Joaquín
    Jones, Nic
    Hidalgo, Elena
    Affiliation
    Cell Signalling Unit, Department de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain.
    Issue Date
    2002-07
    
    Metadata
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    Abstract
    During the last decade, much has been learnt about the mechanisms by which oxidative stress is perceived by aerobic organisms. The Schizosaccharomyces pombe Pap1 protein is a transcription factor localized at the cytoplasm, which accumulates in the nucleus in response to different inducers, such as the pro-oxidant hydrogen peroxide (H2O2) or the glutathione-depleting agent diethylmaleate (DEM). As described for other H2O2 sensors, our genetic data indicates that H2O2 reversibly oxidizes two cysteine residues in Pap1 (Cys278 and Cys501). Surprisingly, our studies demonstrate that DEM generates a non-reversible modification of at least two cysteine residues located in or close to the nuclear export signal of Pap1 (Cys523 and Cys532). This modification impedes the interaction of the nuclear exporter Crm1 with the nuclear export signal located at the carboxy-terminal domain of Pap1. Mass spectrometry data suggest that DEM binds to the thiol groups of the target cysteine residues through the formation of a thioether. Here we show that DEM triggers Pap1 nuclear accumulation by a novel molecular mechanism.
    Citation
    Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues. 2002, 45 (1):243-54 Mol. Microbiol.
    Journal
    Molecular Microbiology
    URI
    http://hdl.handle.net/10541/83564
    DOI
    10.1046/j.1365-2958.2002.03020.x
    PubMed ID
    12100563
    Type
    Article
    Language
    en
    ISSN
    0950-382X
    ae974a485f413a2113503eed53cd6c53
    10.1046/j.1365-2958.2002.03020.x
    Scopus Count
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    All Paterson Institute for Cancer Research

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