AffiliationThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
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AbstractThe budding yeast mitotic exit network (MEN) is a signal transduction cascade that controls exit from mitosis by facilitating the release of the cell cycle phosphatase Cdc14 from the nucleolus. The G protein Tem1 regulates MEN activity. The Tem1 guanine nucleotide exchange factor (GEF) Lte1 associates with the cortex of the bud and activates the MEN upon the formation of an anaphase spindle. Thus, the cell cortex has an important but ill-defined role in MEN regulation. Here, we describe a network of conserved cortical cell polarity proteins that have key roles in mitotic exit. The Rho-like GTPase Cdc42, its GEF Cdc24 and its effector Cla4 [a member of the p21-activated kinases (PAKs)] control the initial binding and activation of Lte1 to the bud cortex. Moreover, Cdc24, Cdc42 and Ste20, another PAK, probably function parallel to Lte1 in facilitating mitotic exit. Finally, the cell polarity proteins Kel1 and Kel2 are present in complexes with both Lte1 and Tem1, and negatively regulate mitotic exit.
CitationA role for cell polarity proteins in mitotic exit. 2002, 21 (18):4851-62 EMBO J.
JournalThe EMBO Journal
- Control of Lte1 localization by cell polarity determinants and Cdc14.
- Authors: Seshan A, Bardin AJ, Amon A
- Issue date: 2002 Dec 23
- Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast.
- Authors: Yoshida S, Ichihashi R, Toh-e A
- Issue date: 2003 Jun 9
- Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2.
- Authors: Höfken T, Schiebel E
- Issue date: 2004 Jan 19
- Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae.
- Authors: Jensen S, Geymonat M, Johnson AL, Segal M, Johnston LH
- Issue date: 2002 Dec 15
- Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast.
- Authors: Gulli MP, Jaquenoud M, Shimada Y, Niederhäuser G, Wiget P, Peter M
- Issue date: 2000 Nov