Genetic chemoprotection with mutant O6-alkylguanine-DNA-alkyltransferases.
Affiliation
CRC Gene Therapy Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.Issue Date
2002-02
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One of the main barriers to more efficacious use of modern chemotherapeutic agents, is the collateral toxicity exhibited in normal, highly proliferative tissues, primarily the haemopoietic, gastrointestinal and pulmonary tissues. Drug resistance of tumours to these drugs compounds this problem. This review discusses the role of O6-alkylguanine-DNA alkyltransferase (ATase) in conferring protection against O6-alkylating agents in normal tissue, focusing mainly on the haemopoietic compartment. The development of mutant forms of ATase, which are resistant to the effects of soluble analogues of O6-alkylation such as O6-benzylguanine, is examined and the gene therapy approach of combining these two strategies to confer chemoprotection to vulnerable tissues whilst sensitising malignant tissue is reviewed.Citation
Genetic chemoprotection with mutant O6-alkylguanine-DNA-alkyltransferases. 2002, 2 (1):1-8notCurr Gene TherJournal
Current Gene TherapyDOI
10.2174/1566523023348255PubMed ID
12108970Type
ArticleLanguage
enISSN
1566-5232ae974a485f413a2113503eed53cd6c53
10.2174/1566523023348255
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