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    Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex.

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    Authors
    Scharfenberger, Maren
    Ortiz, Jennifer
    Grau, Nicole
    Janke, Carsten
    Schiebel, Elmar
    Lechner, Johannes
    Affiliation
    Biochemie-Zentrum Heidelberg Ruprecht-Karls Universität, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
    Issue Date
    2003-12-15
    
    Metadata
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    Abstract
    We identified a physical complex consisting of Mtw1p, an established kinetochore protein, with Nnf1p, Nsl1p and Dsn1p and have demonstrated that Nnf1p, Nsl1p and Dsn1p localize to the Saccharomyces cerevisiae kinetochore. When challenged prior to metaphase, the temperature-sensitive mutants nsl1-16 and nsl1-42 as well as Nsl1p-depleted cells failed to establish a bipolar spindle-kinetochore interaction and executed monopolar segregation of sister chromatids. In contrast, an nsl1-16 defect could not be evoked after the establishment of bipolarity. The observed phenotype is characteristic of that of mutants with defects in the protein kinase Ipl1p or components of the Dam-Duo kinetochore complex. However nsl1 mutants did not exhibit a defect in microtubule-kinetochore untethering as the ipl1-321 mutant does. Instead, they exhibited a severe defect in the kinetochore localization of the Dam-Duo complex suggesting this to be the cause for the failure of nsl1 cells to establish bipolarity. Moreover the analysis of Nsl1p-depleted cells indicated that Nsl1p is required for the spindle checkpoint and kinetochore integrity.
    Citation
    Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. 2003, 22 (24):6584-97 EMBO J.
    Journal
    The EMBO Journal
    URI
    http://hdl.handle.net/10541/82358
    DOI
    10.1093/emboj/cdg636
    PubMed ID
    14657030
    Type
    Article
    Language
    en
    ISSN
    0261-4189
    ae974a485f413a2113503eed53cd6c53
    10.1093/emboj/cdg636
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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