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    Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo.

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    Authors
    Kanemaki, Masato
    Sanchez-Diaz, Alberto
    Gambus, Agnieszka
    Labib, Karim
    Affiliation
    Cancer Research UK, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.
    Issue Date
    2003-06-12
    
    Metadata
    Show full item record
    Abstract
    Evolutionarily diverse eukaryotic cells share many conserved proteins of unknown function. Some are essential for cell viability, emphasising their importance for fundamental processes of cell biology but complicating their analysis. We have developed an approach to the large-scale characterization of such proteins, based on conditional and rapid degradation of the target protein in vivo, so that the immediate consequences of bulk protein depletion can be examined. Budding yeast strains have been constructed in which essential proteins of unknown function have been fused to a 'heat-inducible-degron' cassette that targets the protein for proteolysis at 37 degrees C (ref. 4). By screening the collection for defects in cell-cycle progression, here we identify three DNA replication factors that interact with each other and that have uncharacterized homologues in human cells. We have used the degron strains to show that these proteins are required for the establishment and normal progression of DNA replication forks. The degron collection could also be used to identify other, essential, proteins with roles in many other processes of eukaryotic cell biology.
    Citation
    Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. 2003, 423 (6941):720-4 Nature
    Journal
    Nature
    URI
    http://hdl.handle.net/10541/82357
    DOI
    10.1038/nature01692
    PubMed ID
    12768207
    Type
    Article
    Language
    en
    ISSN
    0028-0836
    ae974a485f413a2113503eed53cd6c53
    10.1038/nature01692
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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