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    Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.

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    Authors
    Maekawa, Hiromi
    Usui, Takeo
    Knop, Michael
    Schiebel, Elmar
    Affiliation
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
    Issue Date
    2003-02-03
    
    Metadata
    Show full item record
    Abstract
    The budding yeast spindle aligns along the mother- bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1, mediates contacts of CMs with the cortex of the daughter cell, the bud. Here we established a novel series of events that target Kar9 to the bud cortex. First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9. Thirdly, Kar9 and Cdc28 then move from the SPB to the plus end of CMs directed towards the bud. This movement is dependent upon the microtubule motor protein Kip2. Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles.
    Citation
    Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. 2003, 22 (3):438-49 EMBO J.
    Journal
    The EMBO Journal
    URI
    http://hdl.handle.net/10541/82299
    DOI
    10.1093/emboj/cdg063
    PubMed ID
    12554645
    Type
    Article
    Language
    en
    ISSN
    0261-4189
    ae974a485f413a2113503eed53cd6c53
    10.1093/emboj/cdg063
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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