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dc.contributor.authorHeighway, Jim
dc.contributor.authorMargison, Geoffrey P
dc.contributor.authorSantibanez-Koref, Mauro F
dc.date.accessioned2009-09-23T10:25:41Z
dc.date.available2009-09-23T10:25:41Z
dc.date.issued2003-10
dc.identifier.citationThe alleles of the DNA repair gene O6-alkylguanine-DNA alkyltransferase are expressed at different levels in normal human lung tissue. 2003, 24 (10):1691-4 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid12896905
dc.identifier.doi10.1093/carcin/bgg124
dc.identifier.urihttp://hdl.handle.net/10541/82275
dc.description.abstractO6-Alkylguanine-DNA alkyltransferase (MGMT) confers resistance to many of the mutagenic and toxic effects of certain classes of alkylating agents by repairing the DNA lesions responsible. The levels of expression of this protein are of interest in relation to the prevention and treatment of cancer in man. They vary widely between individuals, and the basis of this variation is not understood. RT-PCR-RFLP analysis of mRNA from normal human lung tissue reveals that the two MGMT alleles are frequently expressed at different levels, indicating that there is a genetic component to inter-individual variation of MGMT levels and that at least some of this variation maps close to or within the MGMT locus.
dc.language.isoenen
dc.subject.meshAlleles
dc.subject.meshDNA Repair
dc.subject.meshHumans
dc.subject.meshLung
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.subject.meshRNA, Messenger
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshTranscription, Genetic
dc.titleThe alleles of the DNA repair gene O6-alkylguanine-DNA alkyltransferase are expressed at different levels in normal human lung tissue.en
dc.typeArticleen
dc.contributor.departmentRoy Castle International Centre for Lung Cancer Research, 200 London Road, Liverpool L3 9TA, UK.en
dc.identifier.journalCarcinogenesisen
html.description.abstractO6-Alkylguanine-DNA alkyltransferase (MGMT) confers resistance to many of the mutagenic and toxic effects of certain classes of alkylating agents by repairing the DNA lesions responsible. The levels of expression of this protein are of interest in relation to the prevention and treatment of cancer in man. They vary widely between individuals, and the basis of this variation is not understood. RT-PCR-RFLP analysis of mRNA from normal human lung tissue reveals that the two MGMT alleles are frequently expressed at different levels, indicating that there is a genetic component to inter-individual variation of MGMT levels and that at least some of this variation maps close to or within the MGMT locus.


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