• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    The conserved Nup107-160 complex is critical for nuclear pore complex assembly.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Walther, Tobias C
    Alves, Annabelle
    Pickersgill, Helen
    Loïodice, Isabelle
    Hetzer, Martin
    Galy, Vincent
    Hülsmann, Bastian B
    Köcher, Thomas
    Wilm, Matthias
    Allen, Terence D
    Mattaj, Iain W
    Doye, Valérie
    Show allShow less
    Affiliation
    EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
    Issue Date
    2003-04-18
    
    Metadata
    Show full item record
    Abstract
    Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.
    Citation
    The conserved Nup107-160 complex is critical for nuclear pore complex assembly. 2003, 113 (2):195-206 Cell
    Journal
    Cell
    URI
    http://hdl.handle.net/10541/82256
    PubMed ID
    12705868
    Type
    Article
    Language
    en
    ISSN
    0092-8674
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly.
    • Authors: Franz C, Walczak R, Yavuz S, Santarella R, Gentzel M, Askjaer P, Galy V, Hetzer M, Mattaj IW, Antonin W
    • Issue date: 2007 Feb
    • Cell cycle-dependent differences in nuclear pore complex assembly in metazoa.
    • Authors: Doucet CM, Talamas JA, Hetzer MW
    • Issue date: 2010 Jun 11
    • ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.
    • Authors: Rasala BA, Orjalo AV, Shen Z, Briggs S, Forbes DJ
    • Issue date: 2006 Nov 21
    • Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
    • Authors: Harel A, Orjalo AV, Vincent T, Lachish-Zalait A, Vasu S, Shah S, Zimmerman E, Elbaum M, Forbes DJ
    • Issue date: 2003 Apr
    • NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts.
    • Authors: Baur T, Ramadan K, Schlundt A, Kartenbeck J, Meyer HH
    • Issue date: 2007 Aug 15
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.