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dc.contributor.authorStringer, Sally E
dc.contributor.authorNelson, Matthew S
dc.contributor.authorGupta, Pankaj
dc.date.accessioned2009-09-23T10:22:54Z
dc.date.available2009-09-23T10:22:54Z
dc.date.issued2003-03-15
dc.identifier.citationIdentification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs. 2003, 101 (6):2243-5 Blooden
dc.identifier.issn0006-4971
dc.identifier.pmid12406885
dc.identifier.doi10.1182/blood-2002-08-2588
dc.identifier.urihttp://hdl.handle.net/10541/82255
dc.description.abstractWe previously showed that heparan sulfate (HS) is required for in vitro cytokine + chemokine-mediated maintenance of primitive human hematopoietic progenitors. However, HS preparations are mixtures of polysaccharide chains of varying size, structure, and protein-binding abilities. Therefore, we examined whether the long-term culture-initiating cells (LTC-IC) supportive capability of HS is attributable to an oligosaccharide of defined length and protein-binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-IC maintenance in the presence of low-dose cytokines and a single chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), was examined. LTC-IC supportive capability of HS oligosaccharides correlated directly with size and MIP-1alpha binding ability. A specific MIP-1alpha-binding HS oligosaccharide preparation of M(r) 10 kDa that optimally supported LTC-IC maintenance was identified. This oligosaccharide had the structure required for MIP-1alpha binding, which we have recently described. The present study defines the minimum size and structural features of LTC-IC supportive HS.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34
dc.subject.meshBone Marrow Cells
dc.subject.meshCells, Cultured
dc.subject.meshChemokine CCL3
dc.subject.meshChemokine CCL4
dc.subject.meshHLA-DR Antigens
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHeparitin Sulfate
dc.subject.meshHumans
dc.subject.meshMacrophage Inflammatory Proteins
dc.subject.meshOligosaccharides
dc.subject.meshProtein Binding
dc.titleIdentification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalBlooden
html.description.abstractWe previously showed that heparan sulfate (HS) is required for in vitro cytokine + chemokine-mediated maintenance of primitive human hematopoietic progenitors. However, HS preparations are mixtures of polysaccharide chains of varying size, structure, and protein-binding abilities. Therefore, we examined whether the long-term culture-initiating cells (LTC-IC) supportive capability of HS is attributable to an oligosaccharide of defined length and protein-binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-IC maintenance in the presence of low-dose cytokines and a single chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), was examined. LTC-IC supportive capability of HS oligosaccharides correlated directly with size and MIP-1alpha binding ability. A specific MIP-1alpha-binding HS oligosaccharide preparation of M(r) 10 kDa that optimally supported LTC-IC maintenance was identified. This oligosaccharide had the structure required for MIP-1alpha binding, which we have recently described. The present study defines the minimum size and structural features of LTC-IC supportive HS.


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