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    Identification of a putative intestinal stem cell and early lineage marker; musashi-1.

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    Authors
    Potten, Christopher S
    Booth, Catherine
    Tudor, Gregory L
    Booth, Dawn
    Brady, Ged
    Hurley, Patricia
    Ashton, Garry
    Clarke, Robert B
    Sakakibara, Shin-ichi
    Okano, Hideyuki
    Affiliation
    Epithelial Biology Department, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. c.potten@epistem.co.uk
    Issue Date
    2003-01
    
    Metadata
    Show full item record
    Abstract
    There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / - ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive. In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.
    Citation
    Identification of a putative intestinal stem cell and early lineage marker; musashi-1. 2003, 71 (1):28-41 Differentiation
    Journal
    Differentiation
    URI
    http://hdl.handle.net/10541/82128
    PubMed ID
    12558601
    Type
    Article
    Language
    en
    ISSN
    0301-4681
    Collections
    All Paterson Institute for Cancer Research

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