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The human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence.

dc.contributor.authorSlater, Nicholas J
dc.contributor.authorYamaguchi, Masafumi
dc.contributor.authorRothwell, Dominic G
dc.contributor.authorBaker, Patrick
dc.contributor.authorHeyworth, Clare M
dc.contributor.authorChopra, Rajesh
dc.date.accessioned2009-09-22T15:11:14Z
dc.date.available2009-09-22T15:11:14Z
dc.date.issued2003-07
dc.identifier.citationThe human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence. 2003, 122 (1):150-8 Br. J. Haematol.en
dc.identifier.issn0007-1048
dc.identifier.pmid12823357
dc.identifier.urihttp://hdl.handle.net/10541/82125
dc.description.abstractA number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the alpha1 and alpha2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of alpha1 or alpha2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-alpha2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-alpha1-expressing cells.
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAlternative Splicing
dc.subject.meshAnimals
dc.subject.meshAntigens, CD34
dc.subject.meshCell Differentiation
dc.subject.meshCell Line
dc.subject.meshErythroid Precursor Cells
dc.subject.meshGene Expression Regulation
dc.subject.meshHematopoiesis
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshProtein Isoforms
dc.subject.meshRNA, Messenger
dc.subject.meshReceptors, Granulocyte-Macrophage Colony-Stimulating Factor
dc.titleThe human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence.en
dc.typeArticleen
dc.contributor.departmentStem Cell and Leukaemia Biology Group, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Haematologyen
html.description.abstractA number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the alpha1 and alpha2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of alpha1 or alpha2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-alpha2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-alpha1-expressing cells.


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