• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Role of proteolytic enzymes in human prostate bone metastasis formation: in vivo and in vitro studies.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Hart, Claire A
    Scott, Linda J
    Bagley, Steven
    Bryden, A A G
    Clarke, Noel W
    Lang, Shona H
    Affiliation
    Cancer Research UK - Group of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. chart@picr.man.ac.uk
    Issue Date
    2002-04-08
    
    Metadata
    Show full item record
    Abstract
    Prostate cancers ability to invade and grow in bone marrow stroma is thought to be due in part to degradative enzymes. The formation of prostate skeletal metastases have been reproduced in vitro by growing co-cultures of prostatic epithelial cells in bone marrow stroma. Expression of urokinase plasminogen activator, matrix metalloproteinase 1 and 7 by prostatic epithelial cells were identified using immunocytochemistry. Also, in vivo tissue sections from human prostatic bone marrow metastases were stained. To establish the role of these enzymes on colony formation, inhibitory antibodies directed against urokinase plasminogen activator, matrix metalloproteinase 1 and matrix metalloproteinase 7 were added into primary prostatic epithelial cells and bone marrow stroma co-cultures. All prostatic epithelial cell cultures stained positively for matrix metalloproteinase 1, matrix metalloproteinase 7 and urokinase plasminogen activator. Generally prostatic epithelial cells derived from malignant tissues showed increased staining in comparison to epithelia derived from non-malignant tissue. In agreement with in vitro co-cultures, the in vivo tissue sections of prostate bone marrow metastases showed positive staining for all three enzymes. Inhibition studies demonstrated that blocking matrix metalloproteinase 1, matrix metalloproteinase 7 and urokinase plasminogen activator function reduced the median epithelial colony area significantly in bone marrow stroma co-cultures in vitro. Using a human ex-vivo model we have shown that matrix metalloproteinase 1, matrix metalloproteinase 7 and urokinase plasminogen activator play an important role in the establishment of prostatic epithelial cells within bone marrow.
    Citation
    Role of proteolytic enzymes in human prostate bone metastasis formation: in vivo and in vitro studies. 2002, 86 (7):1136-42 Br. J. Cancer
    Journal
    British Journal of Cancer
    URI
    http://hdl.handle.net/10541/81264
    DOI
    10.1038/sj.bjc.6600207
    PubMed ID
    11953862
    Type
    Article
    Language
    en
    ISSN
    0007-0920
    ae974a485f413a2113503eed53cd6c53
    10.1038/sj.bjc.6600207
    Scopus Count
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.
    • Authors: Lang SH, Clarke NW, George NJ, Allen TD, Testa NG
    • Issue date: 1998 Feb 15
    • Urokinase-type plasminogen activator: a paracrine factor regulating the bioavailability of IGFs in PA-III cell-induced osteoblastic metastases.
    • Authors: Koutsilieris M, Frenette G, Lazure C, Lehoux JG, Govindan MV, Polychronakos C
    • Issue date: 1993 Mar-Apr
    • Aspirin Inhibits IKK-β-mediated Prostate Cancer Cell Invasion by Targeting Matrix Metalloproteinase-9 and Urokinase-Type Plasminogen Activator.
    • Authors: Shi C, Zhang N, Feng Y, Cao J, Chen X, Liu B
    • Issue date: 2017
    • Plasminogen activator activity in bone metastases of prostatic carcinomas as compared to primary tumors.
    • Authors: Kirchheimer JC, Pflüger H, Ritschl P, Hienert G, Binder BR
    • Issue date: 1985
    • Plasminogen activator and matrix metalloproteinase production and extracellular matrix degradation by rat prostate cancer cells in vitro: correlation with metastatic behavior in vivo.
    • Authors: Quax PH, de Bart AC, Schalken JA, Verheijen JH
    • Issue date: 1997 Aug 1
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.