• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Development of a novel rapid assay to assess the fidelity of DNA double-strand-break repair in human tumour cells.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Collis, S J
    Sangar, Vijay K
    Tighe, A
    Roberts, Stephen A
    Clarke, Noel W
    Hendry, Jolyon H
    Margison, Geoffrey P
    Affiliation
    CRC Experimental Radiation Oncology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
    Issue Date
    2002-01-15
    
    Metadata
    Show full item record
    Abstract
    Cellular survival following ionising radiation-mediated damage is primarily a function of the ability to successfully detect and repair DNA double-strand breaks (DSBs). Previous studies have demonstrated that radiosensitivity, determined as a reduction in colony forming ability in vitro, may be related to the incorrect repair (misrepair) of DSBs. The novel rapid dual fluorescence (RDF) assay is a plasmid-based reporter system that rapidly assesses the correct rejoining of a restriction-enzyme produced DSBs within transfected cells. We have utilised this novel assay to determine the fidelity of DSB repair in the prostate tumour cell line LNCaP, the bladder tumour cell line MGH-U1 and a radiosensitive subclone S40b. The two bladder cell lines have been shown in previous studies to differ in their ability to correctly repair plasmids containing a single DSB. Using the RDF assay we found that a substantial portion of LNCaP cells [80.4 +/- 5.3(standard error)%] failed to reconstitute reporter gene expression; however, there was little difference in this measure of DSB repair fidelity between the two bladder cell lines (48.3 +/- 3.5% for MGH-U1; 39.9 +/- 8.2% for S40b). The RDF assay has potential to be developed to study the relationship between DSB repair fidelity and radiosensitivity as well as the mechanisms associated with this type of repair defect.
    Citation
    Development of a novel rapid assay to assess the fidelity of DNA double-strand-break repair in human tumour cells. 2002, 30 (2):E1 Nucleic Acids Res.
    Journal
    Nucleic Acids Research
    URI
    http://hdl.handle.net/10541/81257
    PubMed ID
    11788727
    Type
    Article
    Language
    en
    ISSN
    1362-4962
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • The repair fidelity of restriction enzyme-induced double strand breaks in plasmid DNA correlates with radioresistance in human tumor cell lines.
    • Authors: Powell SN, McMillan TJ
    • Issue date: 1994 Jul 30
    • Differential level of DSB repair fidelity effected by nuclear protein extracts derived from radiosensitive and radioresistant human tumour cells.
    • Authors: Britten RA, Liu D, Kuny S, Allalunis-Turner MJ
    • Issue date: 1997
    • Rejoining of DNA double-strand breaks after the introduction of chromosome 11 into a radiosensitive bladder carcinoma cell line.
    • Authors: Hofseth LJ, Tsang SS, Rosin MP
    • Issue date: 1997 Jan 31
    • Radiation-induced DNA double-strand break rejoining in human tumour cells.
    • Authors: Núñez MI, Villalobos M, Olea N, Valenzuela MT, Pedraza V, McMillan TJ, Ruiz de Almodóvar JM
    • Issue date: 1995 Feb
    • A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
    • Authors: Powell SN, Whitaker SJ, Edwards SM, McMillan TJ
    • Issue date: 1992 Jun
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.