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    Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study.

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    Authors
    Gazi, Ehsan
    Dwyer, John
    Gardner, Peter
    Ghanbari-Siahkali, A
    Wade, A P
    Miyan, J
    Lockyer, Nicholas P
    Vickerman, John C
    Clarke, Noel W
    Shanks, Jonathan H
    Scott, Linda J
    Hart, Claire A
    Brown, Michael D
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    Affiliation
    Department of Chemistry, UMIST, PO Box 88, Manchester M60 1QD, UK.
    Issue Date
    2003-09
    
    Metadata
    Show full item record
    Abstract
    Fourier transform infrared (FTIR) microspectroscopy has been applied to a study of prostate cancer cell lines derived from different metastatic sites and to tissue from benign prostate and Gleason-graded malignant prostate tissue. Paraffin-embedded tissue samples were analysed by FTIR, after mounting onto a BaF(2) plate and subsequent removal of wax using Citroclear followed by acetone. Cell lines were analysed as aliquots of cell suspension held between two BaF(2) plates. It was found that the ratio of peak areas at 1030 and 1080 cm(-1), corresponding to the glycogen and phosphate vibrations respectively, suggests a potential method for the differentiation of benign from malignant cells. The use of this ratio in association with FTIR spectral imaging provides a basis for estimating areas of malignant tissue within defined regions of a specimen. Initial chemometric treatment of FTIR spectra, using the linear discriminant algorithm, demonstrates a promising method for the classification of benign and malignant tissue and the separation of Gleason-graded CaP spectra. Using the principle component analysis, this study has achieved for the first time the separation of FTIR spectra of prostate cancer cell lines derived from different metastatic sites.
    Citation
    Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study. 2003, 201 (1):99-108 J. Pathol.
    Journal
    The Journal of Pathology
    URI
    http://hdl.handle.net/10541/79121
    DOI
    10.1002/path.1421
    PubMed ID
    12950022
    Type
    Article
    Language
    en
    ISSN
    0022-3417
    ae974a485f413a2113503eed53cd6c53
    10.1002/path.1421
    Scopus Count
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

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