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dc.contributor.authorRenehan, Andrew G
dc.contributor.authorJones, Jenny
dc.contributor.authorO'Dwyer, Sarah T
dc.contributor.authorShalet, Stephen M
dc.date.accessioned2009-08-27T15:31:05Z
dc.date.available2009-08-27T15:31:05Z
dc.date.issued2003-12
dc.identifier.citationDetermination of IGF-I, IGF-II, IGFBP-2, and IGFBP-3 levels in serum and plasma: comparisons using the Bland-Altman method. 2003, 13 (6):341-6 Growth Horm. IGF Res.en
dc.identifier.issn1096-6374
dc.identifier.pmid14624768
dc.identifier.doi10.1016/S1096-6374(03)00112-6
dc.identifier.urihttp://hdl.handle.net/10541/78969
dc.description.abstractThe measurement of circulating insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs) have significant implications in the risk assessment of various diseases (e.g. cancer) and growth abnormalities. It is often assumed that values measured in serum and plasma are interchangeable. This study challenges this assumption by comparing determinants using the Bland-Altman method. Blood was obtained from 47 healthy volunteers (age 21-72 years) in serum, heparin plasma and EDTA plasma, and IGF-I, IGF-II, IGFBP-2, and IGFBP-3 measured, and results compared. Mean values for IGF-I, IGF-II, IGFBP-2 and IGFBP-3 determined in all three media were generally comparable; correlations were generally strong and significant (P<0.001). However, the Bland-Altman plots revealed significant lack of agreement for many analytes measured in EDTA plasma compared with serum and heparin plasma. Additionally, the ranges of the limits of agreement were consistently greater for EDTA plasma compared with the other two methods. These findings emphasize the need to standardize methods of collecting blood samples in future epidemiological studies and trials.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBlood Specimen Collection
dc.subject.meshEdetic Acid
dc.subject.meshFemale
dc.subject.meshHeparin
dc.subject.meshHumans
dc.subject.meshInsulin-Like Growth Factor Binding Protein 2
dc.subject.meshInsulin-Like Growth Factor Binding Protein 3
dc.subject.meshInsulin-Like Growth Factor I
dc.subject.meshInsulin-Like Growth Factor II
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.titleDetermination of IGF-I, IGF-II, IGFBP-2, and IGFBP-3 levels in serum and plasma: comparisons using the Bland-Altman method.en
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. arenehan@picr.man.ac.uken
dc.identifier.journalGrowth Hormone & IGF Researchen
html.description.abstractThe measurement of circulating insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs) have significant implications in the risk assessment of various diseases (e.g. cancer) and growth abnormalities. It is often assumed that values measured in serum and plasma are interchangeable. This study challenges this assumption by comparing determinants using the Bland-Altman method. Blood was obtained from 47 healthy volunteers (age 21-72 years) in serum, heparin plasma and EDTA plasma, and IGF-I, IGF-II, IGFBP-2, and IGFBP-3 measured, and results compared. Mean values for IGF-I, IGF-II, IGFBP-2 and IGFBP-3 determined in all three media were generally comparable; correlations were generally strong and significant (P<0.001). However, the Bland-Altman plots revealed significant lack of agreement for many analytes measured in EDTA plasma compared with serum and heparin plasma. Additionally, the ranges of the limits of agreement were consistently greater for EDTA plasma compared with the other two methods. These findings emphasize the need to standardize methods of collecting blood samples in future epidemiological studies and trials.


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