• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    GLUT-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: relationship to pimonidazole binding.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Airley, R
    Loncaster, Juliette A
    Raleigh, James A
    Harris, Adrian L
    Davidson, Susan E
    Hunter, Robin D
    West, Catharine M L
    Stratford, Ian J
    Affiliation
    School of Pharmacy and Chemistry, Liverpool John Moores University, Liverpool, United Kingdom. R.Airley@livjm.ac.uk
    Issue Date
    2003-03-10
    
    Metadata
    Show full item record
    Abstract
    The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the transcription factor HIF-1. Hypoxic cells are known to be radioresistant and chemoresistant, thus, a reliable surrogate marker of hypoxia is desirable to ensure that treatment may be rationally applied. Recently, the HIF-1-regulated proteins Glut-1 and CAIX were validated as intrinsic markers of hypoxia by comparison with pO(2) measured using oxygen electrodes. We compare the expression of Glut-1 and CAIX with the binding of the bioreductive drug hypoxia marker pimonidazole. Pimonidazole was administered to 42 patients with advanced carcinoma of the cervix, 16 hr before biopsy. Sections of single or multiple biopsies were then immunostained for Glut-1 and CAIX, and the area of staining scored by eye, using a "field-by-field" semi-quantitative averaging system. Using 1 biopsy only, Glut-1 (r = 0.54, p = <0.001) correlated with the level of pimonidazole binding, and Glut-1 and CAIX expression also correlated significantly (r = 0.40, p = <0.009). Thus, our study has shown that HIF-1 regulated genes have potential for future use as predictors of the malignant changes mediated by hypoxia, and warrant further investigation as indicators of response to cancer therapy.
    Citation
    GLUT-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: relationship to pimonidazole binding. 2003, 104 (1):85-91 Int. J. Cancer
    Journal
    International Journal of Cancer
    URI
    http://hdl.handle.net/10541/78774
    DOI
    10.1002/ijc.10904
    PubMed ID
    12532423
    Type
    Article
    Language
    en
    ISSN
    0020-7136
    ae974a485f413a2113503eed53cd6c53
    10.1002/ijc.10904
    Scopus Count
    Collections
    All Christie Publications

    entitlement

    Related articles

    • Comparison between pimonidazole binding, oxygen electrode measurements, and expression of endogenous hypoxia markers in cancer of the uterine cervix.
    • Authors: Jankovic B, Aquino-Parsons C, Raleigh JA, Stanbridge EJ, Durand RE, Banath JP, MacPhail SH, Olive PL
    • Issue date: 2006 Mar
    • GLUT1 and CAIX as intrinsic markers of hypoxia in bladder cancer: relationship with vascularity and proliferation as predictors of outcome of ARCON.
    • Authors: Hoskin PJ, Sibtain A, Daley FM, Wilson GD
    • Issue date: 2003 Oct 6
    • Hypoxia biomarkers in squamous cell carcinoma of the uterine cervix.
    • Authors: Ellingsen C, Andersen LM, Galappathi K, Rofstad EK
    • Issue date: 2015 Oct 26
    • Metabolic markers in relation to hypoxia; staining patterns and colocalization of pimonidazole, HIF-1α, CAIX, LDH-5, GLUT-1, MCT1 and MCT4.
    • Authors: Rademakers SE, Lok J, van der Kogel AJ, Bussink J, Kaanders JH
    • Issue date: 2011 May 12
    • Reduced expression of hypoxia-inducible factor-1alpha in perinecrotic regions of solid tumors.
    • Authors: Sobhanifar S, Aquino-Parsons C, Stanbridge EJ, Olive P
    • Issue date: 2005 Aug 15
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.