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dc.contributor.authorMassé, Karine
dc.contributor.authorBaldwin, Rebecca J
dc.contributor.authorBarnett, Mark W
dc.contributor.authorJones, Elizabeth A
dc.date.accessioned2009-08-25T11:27:00Z
dc.date.available2009-08-25T11:27:00Z
dc.date.issued2004-12
dc.identifier.citationX-epilectin: a novel epidermal fucolectin regulated by BMP signalling. 2004, 48 (10):1119-29 Int. J. Dev. Biol.en
dc.identifier.issn0214-6282
dc.identifier.pmid15602698
dc.identifier.doi10.1387/ijdb.041892km
dc.identifier.urihttp://hdl.handle.net/10541/78473
dc.description.abstractThis paper reports the cloning and characterisation of a new posterior epidermal marker, X-epilectin, in Xenopus laevis. This gene encodes for a fucolectin, which belongs to the lectin superfamily of carbohydrate binding proteins and specifically binds fucose residues. RT-PCR and in situ hybridisation show that the expression of this gene is switched on during gastrulation and up-regulated during neurula stages and found expressed ubiquitously throughout the epidermis. From tailbud stages, the expression is limited to the dorsal posterior region of the embryo, suggesting that X-epilectin expression is regulated along anteroposterior and dorsoventral gradients during development. In the adult, X-epilectin is mainly expressed in intestinal components, kidney, spinal cord and skin. The effects of growth factors on the regulation of X-epilectin were studied. Change of the fate of animal caps into cement gland or dorsal mesoderm induces a down-regulation of X-epilectin expression in explants treated respectively with ammonium chloride and activin A. We also show that X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway.
dc.language.isoenen
dc.subject.meshActivins
dc.subject.meshAmino Acid Sequence
dc.subject.meshAmmonium Chloride
dc.subject.meshAnimals
dc.subject.meshBase Sequence
dc.subject.meshBone Morphogenetic Protein 4
dc.subject.meshBone Morphogenetic Protein Receptors
dc.subject.meshBone Morphogenetic Proteins
dc.subject.meshCarrier Proteins
dc.subject.meshCell Differentiation
dc.subject.meshCloning, Molecular
dc.subject.meshDNA, Complementary
dc.subject.meshDown-Regulation
dc.subject.meshGene Expression Regulation
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshIn Situ Hybridization
dc.subject.meshInhibin-beta Subunits
dc.subject.meshLectins
dc.subject.meshModels, Genetic
dc.subject.meshMolecular Sequence Data
dc.subject.meshProteins
dc.subject.meshRNA
dc.subject.meshRNA, Messenger
dc.subject.meshReceptors, Growth Factor
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSignal Transduction
dc.subject.meshSkin
dc.subject.meshTime Factors
dc.subject.meshTissue Distribution
dc.subject.meshUp-Regulation
dc.subject.meshXenopus Proteins
dc.subject.meshXenopus laevis
dc.titleX-epilectin: a novel epidermal fucolectin regulated by BMP signalling.en
dc.typeArticleen
dc.contributor.departmentMolecular Physiology, Department of Biological Sciences, Warwick University, Coventry, UK.en
dc.identifier.journalThe International Journal of Developmental Biologyen
html.description.abstractThis paper reports the cloning and characterisation of a new posterior epidermal marker, X-epilectin, in Xenopus laevis. This gene encodes for a fucolectin, which belongs to the lectin superfamily of carbohydrate binding proteins and specifically binds fucose residues. RT-PCR and in situ hybridisation show that the expression of this gene is switched on during gastrulation and up-regulated during neurula stages and found expressed ubiquitously throughout the epidermis. From tailbud stages, the expression is limited to the dorsal posterior region of the embryo, suggesting that X-epilectin expression is regulated along anteroposterior and dorsoventral gradients during development. In the adult, X-epilectin is mainly expressed in intestinal components, kidney, spinal cord and skin. The effects of growth factors on the regulation of X-epilectin were studied. Change of the fate of animal caps into cement gland or dorsal mesoderm induces a down-regulation of X-epilectin expression in explants treated respectively with ammonium chloride and activin A. We also show that X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway.


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