X-epilectin: a novel epidermal fucolectin regulated by BMP signalling.
Affiliation
Molecular Physiology, Department of Biological Sciences, Warwick University, Coventry, UK.Issue Date
2004-12
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Show full item recordAbstract
This paper reports the cloning and characterisation of a new posterior epidermal marker, X-epilectin, in Xenopus laevis. This gene encodes for a fucolectin, which belongs to the lectin superfamily of carbohydrate binding proteins and specifically binds fucose residues. RT-PCR and in situ hybridisation show that the expression of this gene is switched on during gastrulation and up-regulated during neurula stages and found expressed ubiquitously throughout the epidermis. From tailbud stages, the expression is limited to the dorsal posterior region of the embryo, suggesting that X-epilectin expression is regulated along anteroposterior and dorsoventral gradients during development. In the adult, X-epilectin is mainly expressed in intestinal components, kidney, spinal cord and skin. The effects of growth factors on the regulation of X-epilectin were studied. Change of the fate of animal caps into cement gland or dorsal mesoderm induces a down-regulation of X-epilectin expression in explants treated respectively with ammonium chloride and activin A. We also show that X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway.Citation
X-epilectin: a novel epidermal fucolectin regulated by BMP signalling. 2004, 48 (10):1119-29 Int. J. Dev. Biol.Journal
The International Journal of Developmental BiologyDOI
10.1387/ijdb.041892kmPubMed ID
15602698Type
ArticleLanguage
enISSN
0214-6282ae974a485f413a2113503eed53cd6c53
10.1387/ijdb.041892km