AC133+ G0 cells from cord blood show a high incidence of long-term culture-initiating cells and a capacity for more than 100 million-fold amplification of colony-forming cells in vitro.
AuthorsSummers, Yvonne J
Heyworth, Clare M
De Wynter, Erika A
Hart, Claire A
Testa, Nydia G
AffiliationCancer Research UK Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester. firstname.lastname@example.org
MetadataShow full item record
AbstractAC133+ cells may provide an alternative to CD34+ cells as a target for cell expansion and gene therapy protocols. We examined the differences in proliferative potential between cord blood selected for AC133 or CD34 in serum-free, stroma cell-free culture for up to 30 weeks. Because most hemopoietic stem cells reside within the G0/G1 phase of the cell cycle, we combined enrichment according to AC133 or CD34 expression with G0 position in the cell cycle to identify populations enriched for putative stem cells. Our results show that AC133+ G0 cells demonstrated a long-term culture-initiating cell incidence of 1 in 4.2 cells, had a colony-forming cell incidence of 1 in 2.8 cells, were capable of producing 660 million-fold expansion of nucleated cells and 120 million-fold expansion of colony-forming units-granulocyte-macrophage over a period of 30 weeks, and were consistently superior to CD34+ G0 cells according to these parameters. Furthermore, we have shown that AC133+CD34- cells have the ability to generate CD34+ cells in culture, which suggests that at least some AC133+ cells are ancestral to CD34+ cells. We conclude that AC133 isolation provides a better means of selection for primitive hemopoietic cells than CD34 and that, in combination with isolation according to G0 phase of the cell cycle, AC133 isolation identifies a highly enriched population of putative stem cells.
CitationAC133+ G0 cells from cord blood show a high incidence of long-term culture-initiating cells and a capacity for more than 100 million-fold amplification of colony-forming cells in vitro. 2004, 22 (5):704-15 Stem Cells
- Extensive amplification of single cells from CD34+ subpopulations in umbilical cord blood and identification of long-term culture-initiating cells present in two subsets.
- Authors: de Wynter EA, Nadali G, Coutinho LH, Testa NG
- Issue date: 1996 Sep
- In vitro proliferation potential of AC133 positive cells in peripheral blood.
- Authors: Matsumoto K, Yasui K, Yamashita N, Horie Y, Yamada T, Tani Y, Shibata H, Nakano T
- Issue date: 2000
- CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
- Authors: de Wynter EA, Buck D, Hart C, Heywood R, Coutinho LH, Clayton A, Rafferty JA, Burt D, Guenechea G, Bueren JA, Gagen D, Fairbairn LJ, Lord BI, Testa NG
- Issue date: 1998
- Isolation and characterization of human CD34(-)Lin(-) and CD34(+)Lin(-) hematopoietic stem cells using cell surface markers AC133 and CD7.
- Authors: Gallacher L, Murdoch B, Wu DM, Karanu FN, Keeney M, Bhatia M
- Issue date: 2000 May 1
- Human immunodeficiency virus infection of human placental cord blood CD34+AC133+ stem cells and their progeny.
- Authors: Hariharan D, Li Y, Campbell DE, Douglas SD, Starr SE, Ho W
- Issue date: 1999 Nov 20