Tracking mesoderm formation and specification to the hemangioblast in vitro.
AffiliationPaterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, UK.
MetadataShow full item record
AbstractDuring development of the mouse embryo, blood cells are generated from mesodermal precursors at specific times and locations. Using various in vivo and in vitro systems, we are now starting to understand the cascade of molecular events leading to the commitment of mesoderm and the formation of the first blood precursors, the hemangioblast. The in vitro differentiation of embryonic stem (ES) cells has proved to be an invaluable model to study lineage commitment, because one can access and easily manipulate large quantities of early progenitor cells. To help us track mesodermal subpopulations and study their specification toward blood lineages, we have engineered an ES cell line that expresses the green fluorescence protein exclusively in the mesoderm germ layer, under the control of the Brachyury regulatory sequences.
CitationTracking mesoderm formation and specification to the hemangioblast in vitro. 2004, 14 (8):314-7 Trends Cardiovasc. Med.
JournalTrends in Cardiovascular Medicine
- Hemangioblast development and regulation.
- Authors: Choi K
- Issue date: 1998
- Evidence for the hemangioblast.
- Authors: Park C, Ma YD, Choi K
- Issue date: 2005 Sep
- Development of definitive endoderm from embryonic stem cells in culture.
- Authors: Kubo A, Shinozaki K, Shannon JM, Kouskoff V, Kennedy M, Woo S, Fehling HJ, Keller G
- Issue date: 2004 Apr
- The SHB adapter protein is required for normal maturation of mesoderm during in vitro differentiation of embryonic stem cells.
- Authors: Kriz V, Agren N, Lindholm CK, Lenell S, Saldeen J, Mares J, Welsh M
- Issue date: 2006 Nov 10
- Haploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells.
- Authors: Lacaud G, Kouskoff V, Trumble A, Schwantz S, Keller G
- Issue date: 2004 Feb 1