Translocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination.
Affiliation
Academic Unit of Oncology and Haematology, University of Leeds, Leeds, United Kingdom. jamesf@lrf.leeds.ac.ukIssue Date
2004-02
Metadata
Show full item recordAbstract
We describe the characterization of the genomic DNA breakpoints of two multiple myeloma (MM) patients with t(11;14). IGH translocation events are present in many MM tumors, and it is proposed that they occur early in the pathogenesis, based on the assumption that the translocations are simple reciprocal events mediated by errors in class-switch recombination (CSR). We provide evidence from two patients that the translocation event can be more complex, with DNA from chromosome band 11q13 joined to apparently already recombined hybrid (Smu/Sgamma) switch region sequences. In one case, there was also evidence that a further rearrangement had occurred at the t(11;14) recombination site, resulting in an inversion of 40 bp of the 5'Smu flanking sequence. This suggests that primary IGH arrangements in MM may be more complex than previous myeloma models have suggested, but that they essentially occur through illegitimate CSR events.Citation
Translocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination. 2004, 39 (2):151-5 Genes Chromosomes CancerJournal
Genes, Chromosomes & CancerDOI
10.1002/gcc.10304PubMed ID
14695995Type
ArticleLanguage
enISSN
1045-2257ae974a485f413a2113503eed53cd6c53
10.1002/gcc.10304
Scopus Count
Collections
Related articles
- Genomic characterization of the chromosomal breakpoints of t(4;14) of multiple myeloma suggests more than one possible aetiological mechanism.
- Authors: Fenton JA, Pratt G, Rawstron AC, Sibley K, Rothwell D, Yates Z, Dring A, Richards SJ, Ashcroft AJ, Davies FE, Owen RG, Child JA, Morgan GJ
- Issue date: 2003 Feb 20
- Chromosome translocations in multiple myeloma.
- Authors: Bergsagel PL, Kuehl WM
- Issue date: 2001 Sep 10
- Duplication of chromosome arms 9q and 11q: evidence for a novel, 14q32 translocation-independent pathogenetic pathway in multiple myeloma.
- Authors: Liebisch P, Scheck D, Erné SA, Wellmann A, Wendl C, Janczik S, Kolmus S, Kröber A, Einsele H, Straka C, Goldschmidt H, Benner A, Stilgenbauer S, Döhner H
- Issue date: 2005 Jan
- Complex biallelic IGH rearrangements in IgM-expressing Z-138 cell line: Involvement of downstream immunoglobulin class switch recombination.
- Authors: Guikema JE, Fenton JA, de Boer C, Kleiverda K, Brink AA, Raap AK, Estrov Z, Schuuring E, Kluin PM
- Issue date: 2005 Feb
- Translocation and gross deletion breakpoints in human inherited disease and cancer I: Nucleotide composition and recombination-associated motifs.
- Authors: Abeysinghe SS, Chuzhanova N, Krawczak M, Ball EV, Cooper DN
- Issue date: 2003 Sep