Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2.
AffiliationThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Rd., Manchester, M20 4BX, UK.
MetadataShow full item record
AbstractThe guanine nucleotide exchange factor Cdc24, the GTPase Cdc42, and the Cdc42 effectors Cla4 and Ste20, two p21-activated kinases, form a signal transduction cascade that promotes mitotic exit in yeast. We performed a genetic screen to identify components of this pathway. Two related bud cortex-associated Cdc42 effectors, Gic1 and Gic2, were obtained as factors that promoted mitotic exit independently of Ste20. The mitotic exit function of Gic1 was dependent on its activation by Cdc42 and on the release of Gic1 from the bud cortex. Gic proteins became essential for mitotic exit when activation of the mitotic exit network through Cdc5 polo kinase and the bud cortex protein Lte1 was impaired. The mitotic exit defect of cdc5-10 Deltalte1 Deltagic1 Deltagic2 cells was rescued by inactivation of the inhibiting Bfa1-Bub2 GTPase-activating protein. Moreover, Gic1 bound directly to Bub2 and prevented binding of the GTPase Tem1 to Bub2. We propose that in anaphase the Cdc42-regulated Gic proteins trigger mitotic exit by interfering with Bfa1-Bub2 GTPase-activating protein function.
CitationNovel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2. 2004, 164 (2):219-31 J. Cell Biol.
JournalThe Journal of Cell Biology
- Bfa1 can regulate Tem1 function independently of Bub2 in the mitotic exit network of Saccharomyces cerevisiae.
- Authors: Ro HS, Song S, Lee KS
- Issue date: 2002 Apr 16
- Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints.
- Authors: Hu F, Wang Y, Liu D, Li Y, Qin J, Elledge SJ
- Issue date: 2001 Nov 30
- The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit.
- Authors: Wang Y, Hu F, Elledge SJ
- Issue date: 2000 Nov 2
- A role for cell polarity proteins in mitotic exit.
- Authors: Höfken T, Schiebel E
- Issue date: 2002 Sep 16
- Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae.
- Authors: Jensen S, Geymonat M, Johnson AL, Segal M, Johnston LH
- Issue date: 2002 Dec 15