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    Haemangioblast commitment is initiated in the primitive streak of the mouse embryo.

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    Authors
    Huber, Tara L
    Kouskoff, Valerie
    Fehling, H Joerg
    Palis, James
    Keller, Gordon
    Affiliation
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA.
    Issue Date
    2004-12-02
    
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    Abstract
    Haematopoietic and vascular cells are thought to arise from a common progenitor called the haemangioblast. Support for this concept has been provided by embryonic stem (ES) cell differentiation studies that identified the blast colony-forming cell (BL-CFC), a progenitor with both haematopoietic and vascular potential. Using conditions that support the growth of BL-CFCs, we identify comparable progenitors that can form blast cell colonies (displaying haematopoietic and vascular potential) in gastrulating mouse embryos. Cell mixing and limiting dilution analyses provide evidence that these colonies are clonal, indicating that they develop from a progenitor with haemangioblast potential. Embryo-derived haemangioblasts are first detected at the mid-streak stage of gastrulation and peak in number during the neural plate stage. Analysis of embryos carrying complementary DNA of the green fluorescent protein targeted to the brachyury locus demonstrates that the haemangioblast is a subpopulation of mesoderm that co-expresses brachyury (also known as T) and Flk-1 (also known as Kdr). Detailed mapping studies reveal that haemangioblasts are found at highest frequency in the posterior region of the primitive streak, indicating that initial stages of haematopoietic and vascular commitment occur before blood island development in the yolk sac.
    Citation
    Haemangioblast commitment is initiated in the primitive streak of the mouse embryo. 2004, 432 (7017):625-30 Nature
    Journal
    Nature
    URI
    http://hdl.handle.net/10541/78377
    DOI
    10.1038/nature03122
    PubMed ID
    15577911
    Type
    Article
    Language
    en
    ISSN
    1476-4687
    ae974a485f413a2113503eed53cd6c53
    10.1038/nature03122
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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