Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions.
Affiliation
Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. wardcm@hotmail.comIssue Date
2004-02-15
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Mouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated this further by comparing the differentiation characteristics of five independently derived ES cell lines cultured and differentiated under defined conditions. Undifferentiated ES cell lines exhibited similar morphology and antigen/transcript marker expression. However, upon differentiation in monolayer culture by LIF withdrawal, only two of the lines expressed similar germ layer transcript profiles, and these were significantly altered compared to differentiation in serum-supplemented media. Neurofilament-68k was the only transcript marker common to all cell lines, however, induction of neuroectoderm lineages using 1 microM all-trans retinoic acid (RA) resulted in significant variations in cell number and morphology between the lines. Furthermore, neurons were only formed from clones of the two cell lines that exhibited similar transcript profiles, although the morphology was different between the two. We conclude that the independent ES cell lines in this study differ in their response to alterations in culture conditions in vitro, and the use of an appropriate cell line enables relatively homogeneous neuronal populations to be achieved in monolayer culture under defined conditions.Citation
Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions. 2004, 293 (2):229-38 Exp. Cell Res.Journal
Experimental Cell ResearchDOI
10.1016/j.yexcr.2003.10.017PubMed ID
14729460Language
enISSN
0014-4827ae974a485f413a2113503eed53cd6c53
10.1016/j.yexcr.2003.10.017
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