• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Kiseleva, Elena
    Allen, Terence D
    Rutherford, Sandra A
    Bucci, Mirella
    Wente, Susan R
    Goldberg, Martin W
    Affiliation
    Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, UK. m.w.goldberg@durham.ac.uk
    Issue Date
    2004-03
    
    Metadata
    Show full item record
    Abstract
    The nuclear pore complex (NPC) controls transport of macromolecules across the nuclear envelope. It is large and complex but appears to consist of only approximately 30 different proteins despite its mass of > 60MDa. Vertebrate NPC structure has been analyzed by several methods giving a comprehensive architectural model. Despite our knowledge of yeast nucleoporins, structural data is more limited and suggests the basic organization is similar to vertebrates, but may lack some peripheral and other components. Using field emission scanning electron microscopy to probe NPC structure we found that the yeast, like higher eukaryotic, NPCs contain similar peripheral components. We can detect cytoplasmic rings and evidence of nucleoplasmic rings in yeasts. A filamentous basket is present on the nucleoplasmic face and evidence for cytoplasmic filaments is shown. We observed a central structure, possibly the transporter, that which may be linked to the cytoplasmic ring by internal filaments. Immuno-gold labeling suggested that Nup159p may be attached to the cytoplasmic ring, whereas Nup116p may be associated, partly, with the cytoplasmic filaments. Analysis of a Nup57p mutant suggested a role in maintaining the stability of cytoplasmic components of the NPC. We conclude that peripheral NPC components appear similar in yeasts compared to higher organisms and present a revised model for yeast NPC structural composition.
    Citation
    Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments. 2004, 145 (3):272-88 J. Struct. Biol.
    Journal
    Journal of Structural Biology
    URI
    http://hdl.handle.net/10541/78355
    DOI
    10.1016/j.jsb.2003.11.010
    PubMed ID
    14960378
    Type
    Article
    Language
    en
    ISSN
    1047-8477
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.jsb.2003.11.010
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex.
    • Authors: Patel SS, Belmont BJ, Sante JM, Rexach MF
    • Issue date: 2007 Apr 6
    • Activation of ryanodine receptors in the nuclear envelope alters the conformation of the nuclear pore complex.
    • Authors: Erickson ES, Mooren OL, Moore-Nichols D, Dunn RC
    • Issue date: 2004 Dec 1
    • [Nuclear pores: from yeast to higher eukaryotes].
    • Authors: Doye V
    • Issue date: 2002
    • The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.
    • Authors: Walther TC, Pickersgill HS, Cordes VC, Goldberg MW, Allen TD, Mattaj IW, Fornerod M
    • Issue date: 2002 Jul 8
    • Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction.
    • Authors: Gomez-Ospina N, Morgan G, Giddings TH Jr, Kosova B, Hurt E, Winey M
    • Issue date: 2000 Oct
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.