Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas.
Loncaster, Juliette A
Lindegaard, Jacob C
Davidson, Susan E
West, Catharine M L
Hunter, Robin D
Raleigh, James A
AffiliationDepartment of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark.
MetadataShow full item record
AbstractBACKGROUND AND PURPOSE: The measurement of tumour oxygenation using Eppendorf oxygen-sensitive needle electrodes can provide prognostic information but the method is limited to accessible tumours that are suitable for electrode insertion. In this paper the aim was to study the relationship between such physiological measurements of tumour hypoxia and the labelling of tumours with the hypoxia-specific marker pimonidazole. MATERIALS AND METHODS: Assessment of tumour oxygen partial pressure (pO(2)) using an Eppendorf pO(2) histograph and immunohistochemical pimonidazole labelling was carried out in 86 patients with primary cervix carcinomas. Pimonidazole was given as a single injection (0.5 g/m(2) i.v.) and 10-24 h later pO(2) measurements were made and biopsies taken. Tumour oxygenation status was evaluated as the median tumour pO(2) and the fraction of pO(2) values
CitationMeasurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas. 2003, 67 (1):35-44 Radiother Oncol
JournalRadiotherapy and Oncology
- Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma.
- Authors: Nordsmark M, Loncaster J, Chou SC, Havsteen H, Lindegaard JC, Davidson SE, Varia M, West C, Hunter R, Overgaard J, Raleigh JA
- Issue date: 2001 Feb 1
- The prognostic value of pimonidazole and tumour pO2 in human cervix carcinomas after radiation therapy: a prospective international multi-center study.
- Authors: Nordsmark M, Loncaster J, Aquino-Parsons C, Chou SC, Gebski V, West C, Lindegaard JC, Havsteen H, Davidson SE, Hunter R, Raleigh JA, Overgaard J
- Issue date: 2006 Aug
- Oxygen tension in human tumours measured with polarographic needle electrodes and its relationship to vascular density, necrosis and hypoxia.
- Authors: Lyng H, Sundfør K, Rofstad EK
- Issue date: 1997 Aug
- Proliferation and hypoxia in human squamous cell carcinoma of the cervix: first report of combined immunohistochemical assays.
- Authors: Kennedy AS, Raleigh JA, Perez GM, Calkins DP, Thrall DE, Novotny DB, Varia MA
- Issue date: 1997 Mar 1
- Comparison between the comet assay and pimonidazole binding for measuring tumour hypoxia.
- Authors: Olive PL, Durand RE, Raleigh JA, Luo C, Aquino-Parsons C
- Issue date: 2000 Dec