Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas.
Authors
Nordsmark, MarianneLoncaster, Juliette A
Aquino-Parsons, Christina
Chou, Shu-Chuan
Ladekarl, Morten
Havsteen, Hanne
Lindegaard, Jacob C
Davidson, Susan E
Varia, Mahesh
West, Catharine M L
Hunter, Robin D
Overgaard, Jens
Raleigh, James A
Affiliation
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark.Issue Date
2003-04
Metadata
Show full item recordAbstract
BACKGROUND AND PURPOSE: The measurement of tumour oxygenation using Eppendorf oxygen-sensitive needle electrodes can provide prognostic information but the method is limited to accessible tumours that are suitable for electrode insertion. In this paper the aim was to study the relationship between such physiological measurements of tumour hypoxia and the labelling of tumours with the hypoxia-specific marker pimonidazole. MATERIALS AND METHODS: Assessment of tumour oxygen partial pressure (pO(2)) using an Eppendorf pO(2) histograph and immunohistochemical pimonidazole labelling was carried out in 86 patients with primary cervix carcinomas. Pimonidazole was given as a single injection (0.5 g/m(2) i.v.) and 10-24 h later pO(2) measurements were made and biopsies taken. Tumour oxygenation status was evaluated as the median tumour pO(2) and the fraction of pO(2) valuesCitation
Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas. 2003, 67 (1):35-44 Radiother OncolJournal
Radiotherapy and OncologyPubMed ID
12758238Type
ArticleLanguage
enISSN
0167-8140Collections
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