Effects of Schistosoma haematobium infection on drug-metabolizing enzymes in human bladder cancer tissues.
Affiliation
Department of Bioscience and Technology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. sheweita@hotmail.comIssue Date
2004-03-08
Metadata
Show full item recordAbstract
The mixed function oxidase system includes the phase I drug oxidation proteins e.g. aryl hydrocarbon hydroxylase (AHH), N-nitrosodimethylamine-N-demethylase I (NDMA-dI) and cytochrome b5 which metabolize most carcinogens and xenobiotics into less and/or more active intermediates. These were determined in human bladder tissues diagnosed as bladder cancer only (10 samples) and bladder cancer associated with Schistosoma haematobium (12 samples) and normal bladder tissues (12 samples). In addition to the above enzymes, agents involved in Phase II drug metabolism e.g. glutathione and glutathione S-transferase as well as free radicals (detected as thiobarbituric acid-reactive substances, TBARS) were also determined in these tissues samples. AAH and NDMA-dI, cytochrome b5, and glutathione S-transferase activity decreased by 42, 28, 47 and 32%, respectively, in human bladder cancer tissues. In bladder cancer tissues associated with S. haematobium infection NDMA-dI and GST activity decreased further by 65 and 56%, respectively, whereas AHH activity increased by 50% and levels of reduced glutathione also increased by 43% in cancer tissue and by 29% in schistocome infected bladder cancer tissue. The level of free radicals also increased significantly (by 57%) in infected bladder cancer tissue but not at all in non-infected cancer tissue. Alterations in the activity of phase I and II of drug-metabolizing enzymes in human bladder tissues as a result of S. haematobium infection may therefore change the bladder's capacity to detoxify many endogenous compounds and may also potentiate the deleterious effects of bladder carcinogens, (e.g. N-nitrosamines) which are known to be present in relatively large quantities in the bladder of patients with schistosomiasis. The present study thus provides new insights into mechanisms for the genesis of bladder cancer initiated in association with schistosomiasis.Citation
Effects of Schistosoma haematobium infection on drug-metabolizing enzymes in human bladder cancer tissues. 2004, 205 (1):15-21 Cancer Lett.Journal
Cancer LettersDOI
10.1016/j.canlet.2003.09.023PubMed ID
15036656Type
ArticleLanguage
enISSN
0304-3835ae974a485f413a2113503eed53cd6c53
10.1016/j.canlet.2003.09.023
Scopus Count
Collections
Related articles
- Changes in expression and activity of glutathione S-transferase in different organs of schistosoma haematobium-infected hamster.
- Authors: Sheweita SA, Mostafa MH, Ebid F, El-Sayed W
- Issue date: 2003
- Changes in the expression of cytochrome P450 2E1 and the activity of carcinogen-metabolizing enzymes in Schistosoma haematobium-infected human bladder tissues.
- Authors: Sheweita SA, Abu El-Maati MR, El-Shahat FG, Bazeed MA
- Issue date: 2001 Apr 12
- Carcinogen-metabolizing enzymes and insecticides.
- Authors: Sheweita SA
- Issue date: 2004
- Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection.
- Authors: Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Amro el-K, Oikawa S, Ohnishi S, Murata M, Kawanishi S
- Issue date: 2011 Oct 22
- Genetic polymorphism of glutathione-S-transferase (GST-M1 and GST-T1) in schistosomiasis -associated bladder cancer in Egyptian patients.
- Authors: El Nouby KA, Abd El Hameed AH, Negm OE, Hamouda HE, El Gamal OM, Ismail GM
- Issue date: 2008 Dec