The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer.
Authors
Hayward, Daniel GClarke, Robert B
Faragher, Alison J
Pillai, Meenu R
Hagan, Iain M
Fry, Andrew M
Affiliation
Department of Biochemistry, University of Leicester, Leicester, USA.Issue Date
2004-10-15
Metadata
Show full item recordAbstract
Aneuploidy and chromosome instability are common abnormalities in human cancer. Loss of control over mitotic progression, multipolar spindle formation, and cytokinesis defects are all likely to contribute to these phenotypes. Nek2 is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome. Functional studies have implicated Nek2 in regulation of centrosome separation and spindle formation. Here, we present the first study of the protein expression levels of the Nek2 kinase in human cancer cell lines and primary tumors. Nek2 protein is elevated 2- to 5-fold in cell lines derived from a range of human tumors including those of cervical, ovarian, breast, prostate, and leukemic origin. Most importantly, by immunohistochemistry, we find that Nek2 protein is significantly up-regulated in preinvasive in situ ductal carcinomas of the breast as well as in invasive breast carcinomas. Finally, by ectopic expression of Nek2A in immortalized HBL100 breast epithelial cells, we show that increased Nek2 protein leads to accumulation of multinucleated cells with supernumerary centrosomes. These data highlight the Nek2 kinase as novel potential target for chemotherapeutic intervention in breast cancer.Citation
The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer. 2004, 64 (20):7370-6 Cancer Res.Journal
Cancer ResearchDOI
10.1158/0008-5472.CAN-04-0960PubMed ID
15492258Type
ArticleLanguage
enISSN
0008-5472ae974a485f413a2113503eed53cd6c53
10.1158/0008-5472.CAN-04-0960
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