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dc.contributor.authorEyden, Brian P
dc.contributor.authorRadford, John A
dc.contributor.authorShalet, Stephen M
dc.contributor.authorThomas, Non
dc.contributor.authorBrison, Daniel R
dc.contributor.authorLieberman, Brian A
dc.date.accessioned2009-08-21T09:09:25Z
dc.date.available2009-08-21T09:09:25Z
dc.date.issued2004
dc.identifier.citationUltrastructural preservation of ovarian cortical tissue cryopreserved in dimethylsulfoxide for subsequent transplantation into young female cancer patients., 28 (4):239-45 Ultrastruct Patholen
dc.identifier.issn0191-3123
dc.identifier.pmid15693635
dc.identifier.urihttp://hdl.handle.net/10541/78099
dc.description.abstractCytotoxic therapy in young women with cancer may cause loss of ovarian function, and ovarian cryopreservation has been proposed as a means of preserving fertility. The objective of this study is to assess ultrastructural preservation of follicles in frozen-thawed ovarian cortical strips, previously cryopreserved using dimethylsulfoxide and a standard slow-cool/rapid-thaw protocol. Ovarian cortical strips (patients, n=7) were thawed and fixed in glutaraldehyde for epoxy resin embedding electron microscopy according to conventional procedures. Oocytes were generally well preserved, on the basis of plasma-membrane integrity, uniformity of chromatin pattern, mitochondrial and rough endoplasmic reticulum (rER) cisternal integrity, and absence of cytoplasmic and intranuclear clear spaces. Frequently, rER cisternae were distended and intramitochondrial matrices lost. Granulosa cells showed somewhat variable preservation. Some were dark-staining; others exhibited washed-out cytoplasm containing damaged or artifactually expanded organelles. The consistently good preservation of oocytes and the good but more variable preservation of granulosa cells provides scientific validation of one component in the overall clinical procedure of attempting to reestablish fertility in young female patients after cytotoxic therapy for cancer.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshCryopreservation
dc.subject.meshCryoprotective Agents
dc.subject.meshDimethyl Sulfoxide
dc.subject.meshFemale
dc.subject.meshGranulosa Cells
dc.subject.meshHumans
dc.subject.meshMicroscopy, Electron, Transmission
dc.subject.meshOocytes
dc.subject.meshOvarian Follicle
dc.subject.meshOvary
dc.titleUltrastructural preservation of ovarian cortical tissue cryopreserved in dimethylsulfoxide for subsequent transplantation into young female cancer patients.en
dc.typeArticleen
dc.contributor.departmentDepartment of Histopathology, Christie Hospital, Manchester, UK. brian.eyden@christie-tr.nwest.nhs.uken
dc.identifier.journalUltrastructural Pathologyen
html.description.abstractCytotoxic therapy in young women with cancer may cause loss of ovarian function, and ovarian cryopreservation has been proposed as a means of preserving fertility. The objective of this study is to assess ultrastructural preservation of follicles in frozen-thawed ovarian cortical strips, previously cryopreserved using dimethylsulfoxide and a standard slow-cool/rapid-thaw protocol. Ovarian cortical strips (patients, n=7) were thawed and fixed in glutaraldehyde for epoxy resin embedding electron microscopy according to conventional procedures. Oocytes were generally well preserved, on the basis of plasma-membrane integrity, uniformity of chromatin pattern, mitochondrial and rough endoplasmic reticulum (rER) cisternal integrity, and absence of cytoplasmic and intranuclear clear spaces. Frequently, rER cisternae were distended and intramitochondrial matrices lost. Granulosa cells showed somewhat variable preservation. Some were dark-staining; others exhibited washed-out cytoplasm containing damaged or artifactually expanded organelles. The consistently good preservation of oocytes and the good but more variable preservation of granulosa cells provides scientific validation of one component in the overall clinical procedure of attempting to reestablish fertility in young female patients after cytotoxic therapy for cancer.


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