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dc.contributor.authorGuo, Baoqiang
dc.contributor.authorRooney, Paul
dc.contributor.authorSlevin, Mark
dc.contributor.authorLi, Chenggang
dc.contributor.authorParameshwar, Sudeep
dc.contributor.authorLiu, Donghui
dc.contributor.authorKumar, Patricia
dc.contributor.authorBernabeu, Carmelo
dc.contributor.authorKumar, Shant
dc.date.accessioned2009-08-18T16:01:45Z
dc.date.available2009-08-18T16:01:45Z
dc.date.issued2004-08
dc.identifier.citationOverexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival. 2004, 25 (2):285-91 Int. J. Oncol.en
dc.identifier.issn1019-6439
dc.identifier.pmid15254724
dc.identifier.urihttp://hdl.handle.net/10541/77753
dc.description.abstractCD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntigens, CD
dc.subject.meshAntigens, CD29
dc.subject.meshCell Adhesion
dc.subject.meshCell Survival
dc.subject.meshGene Expression
dc.subject.meshHumans
dc.subject.meshMitogen-Activated Protein Kinase 8
dc.subject.meshMyoblasts
dc.subject.meshPhosphorylation
dc.subject.meshRats
dc.subject.meshReceptors, Cell Surface
dc.subject.meshSignal Transduction
dc.subject.meshTransfection
dc.subject.meshUp-Regulation
dc.subject.meshVascular Cell Adhesion Molecule-1
dc.titleOverexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.en
dc.typeArticleen
dc.contributor.departmentLaboratory Medicine Academic Group, The Medical School, University of Manchester, Manchester, M13 9PT, UK. baoqiang.guo@man.ac.uken
dc.identifier.journalInternational Journal of Oncologyen
html.description.abstractCD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.


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