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    Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124.

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    Authors
    Dekker, Bronwen A
    Keen, Heather G
    Shaw, David M
    Disley, Lynn
    Hastings, David L
    Hadfield, John A
    Reader, Andrew J
    Allan, Donald
    Julyan, Peter J
    Watson, Alastair
    Zweit, Jamal
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    Affiliation
    CRUK/UMIST Department of Radiochemical Targeting and Imaging, Paterson Institute for Cancer Research, M20 4BX Manchester, UK. bdekker@picr.man.ac.uk
    Issue Date
    2005-05
    
    Metadata
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    Abstract
    We are interested in imaging cell death in vivo using annexin V radiolabeled with (124)I. In this study, [(124)I]4IB-annexin V and [(124)I]4IB-ovalbumin were made using [(124)I]N-hydroxysuccinimidyl-4-iodobenzoate prepared by iododestannylation of N-hydroxysuccinimidyl-4-(tributylstannyl)benzoate. [(124)I]4IB-annexin V binds to phosphatidylserine-coated microtiter plates and apoptotic Jurkat cells and accumulates in hepatic apoptotic lesions in mice treated with anti-Fas antibody, while [(124)I]4IB-ovalbumin does not. In comparison with (124)I-annexin V, [(124)I]4IB-annexin V has a higher rate of binding to phosphatidylserine in vitro, a higher kidney and urine uptake, a lower thyroid and stomach content uptake, greater plasma stability and a lower rate of plasma clearance. Binding of radioactivity to apoptotic cells relative to normal cells in vitro and in vivo appears to be lower for [(124)I]4IB-annexin V than for (124)I-annexin V.
    Citation
    Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124. 2005, 32 (4):403-13 Nucl. Med. Biol.
    Journal
    Nuclear Medicine and Biology
    URI
    http://hdl.handle.net/10541/76825
    DOI
    10.1016/j.nucmedbio.2005.02.002
    PubMed ID
    15878510
    Type
    Article
    Language
    en
    ISSN
    0969-8051
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.nucmedbio.2005.02.002
    Scopus Count
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    All Christie Publications
    All Paterson Institute for Cancer Research

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